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Published 13 October 2003. doi:10.1083/jcb.200306118
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© The Rockefeller University Press, 0021-9525/2003/10/57 $8.00
The Journal of Cell Biology, Volume 163, Number 1, 57-69

Article

 A role for Yip1p in COPII vesicle biogenesis

Matthew Heidtman1, Catherine Z. Chen2, Ruth N. Collins2 and Charles Barlowe1

1 Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755
2 Department of Molecular Medicine, Cornell University, Veterinary Medical Center, Ithaca, NY 14853

Address correspondence to Charles Barlowe, Dept. of Biochemistry, Dartmouth Medical School, Hanover, NH 03755. Tel.: (603) 650-6516. Fax: (603) 650-1353. email: charles.barlowe{at}dartmouth.edu

Yeast Ypt1p-interacting protein (Yip1p) belongs to a conserved family of transmembrane proteins that interact with Rab GTPases. We encountered Yip1p as a constituent of ER-derived transport vesicles, leading us to hypothesize a direct role for this protein in transport through the early secretory pathway. Using a cell-free assay that recapitulates protein transport from the ER to the Golgi complex, we find that affinity-purified antibodies directed against the hydrophilic amino terminus of Yip1p potently inhibit transport. Surprisingly, inhibition is specific to the COPII-dependent budding stage. In support of this in vitro observation, strains bearing the temperature-sensitive yip1-4 allele accumulate ER membranes at a nonpermissive temperature, with no apparent accumulation of vesicle intermediates. Genetic interaction analyses of the yip1-4 mutation corroborate a function in ER budding. Finally, ordering experiments show that preincubation of ER membranes with COPII proteins decreases sensitivity to anti-Yip1p antibodies, indicating an early requirement for Yip1p in vesicle formation. We propose that Yip1p has a previously unappreciated role in COPII vesicle biogenesis.

Key Words: ER; Golgi; vesicles; coat proteins; trafficking

Abbreviations used in this paper: Erv, ER vesicle; gp{alpha}f, glycopro-{alpha}-factor; Yif1p, Yip1p-interacting factor; Yip1p, Ypt1p-interacting protein.


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