p120 catenin associates with kinesin and facilitates the transport of cadherin-catenin complexes to intercellular junctions

doi:10.1083/jcb.200305137

Published 10 November 2003. doi:10.1083/jcb.200305137

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© The Rockefeller University Press, 0021-9525/2003/11/547 $8.00
The Journal of Cell Biology, Volume 163, Number 3, 547-557

Article

p120 catenin associates with kinesin and facilitates the transport of cadherin–catenin complexes to intercellular junctions

Xinyu Chen¹, Shin-ichiro Kojima², Gary G. Borisy² and Kathleen J. Green¹

¹ Departments of Pathology and Dermatology, R.H. Lurie Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611
² Department of Cell and Molecular Biology, R.H. Lurie Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611

Address correspondence to Kathleen J. Green, Dept. of Pathology, Feinberg School of Medicine, Northwestern University, 303 E. Chicago Ave., Chicago, IL 60611. Tel.: (312) 503-5300. Fax: (312) 503-8240. email: kgreen{at}northwestern.edu

p120 catenin (p120) is a component of adherens junctions andhas been implicated in regulating cadherin-based cell adhesionas well as the activity of Rho small GTPases, but its exactroles in cell–cell adhesion are unclear. Using time-lapseimaging, we show that p120-GFP associates with vesicles andexhibits unidirectional movements along microtubules. Furthermore,p120 forms a complex with kinesin heavy chain through the p120NH₂-terminal head domain. Overexpression of p120, but not anNH₂-terminal deletion mutant deficient in kinesin binding, recruitsendogenous kinesin to N-cadherin. Disruption of the interactionbetween N-cadherin and p120, or the interaction between p120and kinesin, leads to a delayed accumulation of N-cadherin atcell–cell contacts during calcium-initiated junction reassembly.Our analyses identify a novel role of p120 in promoting cellsurface trafficking of cadherins via association and recruitmentof kinesin.

Key Words: Armadillo; adherens junction; N-cadherin; microtubule; trafficking

The online version of this article includes supplemental material.

Abbreviations used in this paper: Arm, armadillo; JMD, juxtamembranedomain; KHC, kinesin heavy chain; KLC, kinesin light chain;MT, microtubule; p120, p120 catenin.

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