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Published 8 December 2003. doi:10.1083/jcb.200310079
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© The Rockefeller University Press, 0021-9525/2003/12/1145 $8.00
The Journal of Cell Biology, Volume 163, Number 5, 1145-1155

Article

 Peritoneal macrophages express both P-selectin and PSGL-1

Boris Tchernychev, Bruce Furie and Barbara C. Furie

Center for Hemostasis, Thrombosis and Vascular Biology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215

Address correspondence to Barbara C. Furie, Center for Hemostasis, Thrombosis and Vascular Biology, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Ave., Boston, MA 02215. Tel.: (617) 667-0620. Fax: (617) 975-5505. email: bfurie1{at}bidmc.harvard.edu

Macrophages, phagocytic cells involved in an early phase of host defense, are known to express the P-selectin ligand, PSGL-1. Heretofore, P-selectin has only been found on platelets and endothelial cells. Here, we demonstrate that peritoneal macrophages isolated by peritoneal lavage of unchallenged mice express P-selectin on the plasma membrane. The peritoneal macrophages synthesize P-selectin, as indicated by metabolic labeling experiments. P-Selectin is constitutively expressed on the extracellular surface of macrophages but is only partially colocalized with PSGL-1. P-Selectin is rapidly translocated from the macrophage plasma membrane to intracellular vesicles and to lysosomes. Peritoneal macrophages assemble into cell strings under flow conditions based upon macrophage–macrophage interactions mediated by P-selectin and PSGL-1. This is the first description of a leukocyte shown to express both P-selectin and PSGL-1.

Key Words: secondary interactions; endocytosis; trafficking; lymphatics


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