Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article Full Text PDF (Full Text) PPT slides of all figures Alert me when this article is cited Citation Map Services Email this article Similar articles in this journal Similar articles in PubMed Alert me to new content in the JCB Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Li, Y. Articles by Camacho, P. Search for Related Content PubMed PubMed Citation Articles by Li, Y. Articles by Camacho, P. Related Collections Related Article Social Bookmarking                      What's this?

Department of Physiology, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229

Address correspondence to Patricia Camacho, Dept. of Physiology, MSC 7756, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr., San Antonio, TX 78229-3900. Tel.: (210) 567-6558. Fax: (210) 567–4410. email: camacho{at}uthscsa.edu

We demonstrated previously that calreticulin (CRT) interacts with the lumenal COOH-terminal sequence of sarco endoplasmic reticulum (ER) calcium ATPase (SERCA) 2b to inhibit Ca²⁺ oscillations. Work from other laboratories demonstrated that CRT also interacts with the ER oxidoreductase, ER protein 57 (also known as ER-60, GRP58; ERp57) during folding of nascent glycoproteins. In this paper, we demonstrate that ERp57 overexpression reduces the frequency of Ca²⁺ oscillations enhanced by SERCA 2b. In contrast, overexpression of SERCA 2b mutants defective in cysteines located in intralumenal loop 4 (L4) increase Ca²⁺ oscillation frequency. In vitro, we demonstrate a Ca²⁺-dependent and -specific interaction between ERp57 and L4. Interestingly, ERp57 does not affect the activity of SERCA 2a or SERCA 2b mutants lacking the CRT binding site. Overexpression of CRT domains that disrupt the interaction of CRT with ERp57 behave as dominant negatives in the Ca²⁺ oscillation assay. Our results suggest that ERp57 modulates the redox state of ER facing thiols in SERCA 2b in a Ca²⁺-dependent manner, providing dynamic control of ER Ca²⁺ homeostasis.

Key Words: calreticulin; calcium ATPases; endoplasmic reticulum; calcium oscillations; glycoprotein folding

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

Related Article

Calcium pumps need a chaperone

Nicole LeBrasseur
J. Cell Biol. 2004 164: 7. [Full Text] [PDF]

This article has been cited by other articles:

• Jeong, W., Lee, D.-Y., Park, S., Rhee, S. G. (2008). ERp16, an Endoplasmic Reticulum-resident Thiol-disulfide Oxidoreductase: BIOCHEMICAL PROPERTIES AND ROLE IN APOPTOSIS INDUCED BY ENDOPLASMIC RETICULUM STRESS. J. Biol. Chem. 283: 25557-25566 [Abstract] [Full Text]  
• Grumolato, L., Ghzili, H., Montero-Hadjadje, M., Gasman, S., Lesage, J., Tanguy, Y., Galas, L., Ait-Ali, D., Leprince, J., Guerineau, N. C., Elkahloun, A. G., Fournier, A., Vieau, D., Vaudry, H., Anouar, Y. (2008). Selenoprotein T is a PACAP-regulated gene involved in intracellular Ca2+ mobilization and neuroendocrine secretion. FASEB J. 22: 1756-1768 [Abstract] [Full Text]  
• Tupling, A. R., Vigna, C., Ford, R. J., Tsuchiya, S. C., Graham, D. A., Denniss, S. G., Rush, J. W. E. (2007). Effects of buthionine sulfoximine treatment on diaphragm contractility and SR Ca2+ pump function in rats. J. Appl. Physiol. 103: 1921-1928 [Abstract] [Full Text]  
• Bruce, J. I. E., Elliott, A. C. (2007). Oxidant-impaired intracellular Ca2+ signaling in pancreatic acinar cells: role of the plasma membrane Ca2+-ATPase. Am. J. Physiol. Cell Physiol. 293: C938-C950 [Abstract] [Full Text]  
• Pastorelli, R., Carpi, D., Campagna, R., Airoldi, L., Pohjanvirta, R., Viluksela, M., Hakansson, H., Boutros, P. C., Moffat, I. D., Okey, A. B., Fanelli, R. (2006). Differential Expression Profiling of the Hepatic Proteome in a Rat Model of Dioxin Resistance: CORRELATION WITH GENOMIC AND TRANSCRIPTOMIC ANALYSES. Mol. Cell. Proteomics 5: 882-894 [Abstract] [Full Text]  
• Porcellini, S., Traggiai, E., Schenk, U., Ferrera, D., Matteoli, M., Lanzavecchia, A., Michalak, M., Grassi, F. (2006). Regulation of peripheral T cell activation by calreticulin.. J. Exp. Med. 203: 461-471 [Abstract] [Full Text]  
• Williams, D. B. (2006). Beyond lectins: the calnexin/calreticulin chaperone system of the endoplasmic reticulum. J. Cell Sci. 119: 615-623 [Abstract] [Full Text]  
• Usachev, Y. M., Marsh, A. J., Johanns, T. M., Lemke, M. M., Thayer, S. A. (2006). Activation of Protein Kinase C in Sensory Neurons Accelerates Ca2+ Uptake into the Endoplasmic Reticulum. J. Neurosci. 26: 311-318 [Abstract] [Full Text]  
• Li, Y., Wright, J. M., Qian, F., Germino, G. G., Guggino, W. B. (2005). Polycystin 2 Interacts with Type I Inositol 1,4,5-Trisphosphate Receptor to Modulate Intracellular Ca2+ Signaling. J. Biol. Chem. 280: 41298-41306 [Abstract] [Full Text]  
• Konstantinov, I. E., Arab, S., Li, J., Coles, J. G., Boscarino, C., Mori, A., Cukerman, E., Dawood, F., Cheung, M. M.H., Shimizu, M., Liu, P. P., Redington, A. N. (2005). The remote ischemic preconditioning stimulus modifies gene expression in mouse myocardium. J. Thorac. Cardiovasc. Surg. 130: 1326-1332 [Abstract] [Full Text]  
• Hetz, C., Russelakis-Carneiro, M., Walchli, S., Carboni, S., Vial-Knecht, E., Maundrell, K., Castilla, J., Soto, C. (2005). The Disulfide Isomerase Grp58 Is a Protective Factor against Prion Neurotoxicity. J. Neurosci. 25: 2793-2802 [Abstract] [Full Text]  
• Verkhratsky, A. (2005). Physiology and Pathophysiology of the Calcium Store in the Endoplasmic Reticulum of Neurons. Physiol. Rev. 85: 201-279 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents