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Published 20 January 2004. doi:10.1083/jcb.200306152
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 164, Number 2, 279-290
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Article

 Atypical protein kinase C (PKC{zeta}/{lambda}) is a convergent downstream target of the insulin-stimulated phosphatidylinositol 3-kinase and TC10 signaling pathways

Makoto Kanzaki1, Silvia Mora1, Joseph B. Hwang2, Alan R. Saltiel2, and Jeffrey E. Pessin1

1 Department of Pharmacological Sciences, State University of New York at Stony Brook, Stony Brook, NY 11794
2 Department of Internal Medicine and Department of Physiology, The University of Michigan Medical Center, Ann Arbor, MI 48109

Address correspondence to Jeffrey E. Pessin, Dept. of Pharmacological Sciences, State University of New York at Stony Brook, Stony Brook, NY 11794-8651. Tel.: (631) 444-3083. Fax: (631) 444-3022. email: pessin{at}pharm.sunysb.edu

Insulin stimulation of adipocytes resulted in the recruitment of atypical PKC (PKC{zeta}/{lambda}) to plasma membrane lipid raft microdomains. This redistribution of PKC{zeta}/{lambda} was prevented by Clostridium difficile toxin B and by cholesterol depletion, but was unaffected by inhibition of phosphatidylinositol (PI) 3-kinase activity. Expression of the constitutively active GTP-bound form of TC10 (TC10Q/75L), but not the inactive GDP-bound mutant (TC10/T31N), targeted PKC{zeta}/{lambda} to the plasma membrane through an indirect association with the Par6–Par3 protein complex. In parallel, insulin stimulation as well as TC10/Q75L resulted in the activation loop phosphorylation of PKC{zeta}. Although PI 3-kinase activation also resulted in PKC{zeta}/{lambda} phosphorylation, it was not recruited to the plasma membrane. Furthermore, insulin-induced GSK-3ß phosphorylation was mediated by both PI 3-kinase–PKB and the TC10–Par6–atypical PKC signaling pathways. Together, these data demonstrate that PKC{zeta}/{lambda} can serve as a convergent downstream target for both the PI 3-kinase and TC10 signaling pathways, but only the TC10 pathway induces a spatially restricted targeting to the plasma membrane.

Key Words: insulin; signal transduction; adipocyte; lipid raft; compartmentalization

Abbreviations used in this paper: CAP, Cbl-associated protein; CRIB, Cdc42/Rac-interacting binding; MßCD, methyl-ß-cyclodextrin; PDK, phosphoinositide-dependent protein kinase; PH, pleckstrin homology; PI, phosphatidylinositol.


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