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Published 20 January 2004. doi:10.1083/jcb.200309134
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 164, Number 2, 313-323
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Article

 Sinuous is a Drosophila claudin required for septate junction organization and epithelial tube size control

Victoria M. Wu1, Joost Schulte2, Alexander Hirschi1, Ulrich Tepass2, and Greg J. Beitel1

1 Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, IL 60208
2 Department of Zoology, University of Toronto, Toronto, Ontario M5S 3G5, Canada

Address correspondence to Greg Beitel, Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Hogan Hall, Rm. 2-100, Northwestern University, 2205 Tech Drive, Evanston, IL 60208-3500. Tel.: (847) 467-7776. Fax: (847) 467-1380. email: beitel{at}northwestern.edu

Epithelial tubes of the correct size and shape are vital for the function of the lungs, kidneys, and vascular system, yet little is known about epithelial tube size regulation. Mutations in the Drosophila gene sinuous have previously been shown to cause tracheal tubes to be elongated and have diameter increases. Our genetic analysis using a sinuous null mutation suggests that sinuous functions in the same pathway as the septate junction genes neurexin and scribble, but that nervana 2, convoluted, varicose, and cystic have functions not shared by sinuous. Our molecular analyses reveal that sinuous encodes a claudin that localizes to septate junctions and is required for septate junction organization and paracellular barrier function. These results provide important evidence that the paracellular barriers formed by arthropod septate junctions and vertebrate tight junctions have a common molecular basis despite their otherwise different molecular compositions, morphologies, and subcellular localizations.

Key Words: tight junction; Drosophila; epithelial cell; trachea; morphogenesis

The online version of this article includes supplemental material.

Abbreviations used in this paper: Arm, Armadillo; conv, convoluted; Cor, coracle; Dlg, Discs large; Dlt, Discs lost; FasIII, Fasciclin III; Mega, megatrachea; Nrv2, nervana 2; Nrx, neurexin; scrib, scribble; TEM, transmission electron microscopy; vari, varicose.


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