JCB logo
Accuri Cytometers
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published 2 February 2004. doi:10.1083/jcb.200306172
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 164, Number 3, 427-439
This Article
Right arrow Full Text
Right arrow PDF (Full Text)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Totsukawa, G.
Right arrow Articles by Matsumura, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Totsukawa, G.
Right arrow Articles by Matsumura, F.
Right arrowPubmed/NCBI databases
*Substance via MeSH
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Article

 Distinct roles of MLCK and ROCK in the regulation of membrane protrusions and focal adhesion dynamics during cell migration of fibroblasts

Go Totsukawa1, Yue Wu2, Yasuharu Sasaki3, David J. Hartshorne2, Yoshihiko Yamakita1, Shigeko Yamashiro1, and Fumio Matsumura1

1 Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, NJ 08855
2 Muscle Biology Group, University of Arizona, Tucson, AZ 85721
3 Department of Pharmacology, School of Pharmaceutical Science, Kitasato University, Tokyo 108-8641, Japan

Address correspondence to Fumio Matsumura, Dept. of Molecular Biology and Biochemistry, Rutgers University, Nelson Labs, Rm. A323, 604 Allison Rd., Piscataway, NJ 08855. Tel.: (732) 445-2838. Fax: (732) 445-4213. email: matsumura{at}mbcl.rutgers.edu

We examined the role of regulatory myosin light chain (MLC) phosphorylation of myosin II in cell migration of fibroblasts. Myosin light chain kinase (MLCK) inhibition blocked MLC phosphorylation at the cell periphery, but not in the center. MLCK-inhibited cells did not assemble zyxin-containing adhesions at the periphery, but maintained focal adhesions in the center. They generated membrane protrusions all around the cell, turned more frequently, and migrated less effectively. In contrast, Rho-associated kinase (ROCK) inhibition blocked MLC phosphorylation in the center, but not at the periphery. ROCK-inhibited cells assembled zyxin-containing adhesions at the periphery, but not focal adhesions in the center. They moved faster and more straight. On the other hand, inhibition of myosin phosphatase increased MLC phosphorylation and blocked peripheral membrane ruffling, as well as turnover of focal adhesions and cell migration. Our results suggest that myosin II activated by MLCK at the cell periphery controls membrane ruffling, and that the spatial regulation of MLC phosphorylation plays critical roles in controlling cell migration of fibroblasts.

Key Words: cell migration; cell polarity; membrane protrusion; MLCK; myosin phosphorylation

The online version of this article includes supplemental material.

Abbreviations used in this paper: BATI, biotin-TAT inhibitor; MLC, myosin light chain; MLCK, myosin light chain kinase; MYPT, myosin phosphatase targeting subunit; ROCK, Rho kinase.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents