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Published 29 March 2004. doi:10.1083/jcb.200311084
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 164, Number 7, 1055-1064
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Article

 The J-domain protein Rme-8 interacts with Hsc70 to control clathrin-dependent endocytosis in Drosophila

Henry C. Chang, Michael Hull, and Ira Mellman

Department of Cell Biology, Ludwig Institute for Cancer Research, Yale University School of Medicine, New Haven, CT 06520

Address correspondence to Ira Mellman, Dept. of Cell Biology, Ludwig Institute for Cancer Research, Yale University School of Medicine, 333 Cedar Street, PO Box 208002, New Haven, CT 06520-8002. Tel.: (203) 785-4303. Fax: (203) 785-4301. email: ira.mellman{at}yale.edu

By screening for mutants exhibiting interactions with a dominant-negative dynamin, we have identified the Drosophila homologue of receptor-mediated endocytosis (Rme) 8, a J-domain–containing protein previously shown to be required for endocytosis in Caenorhabditis elegans. Analysis of Drosophila Rme-8 mutants showed that internalization of Bride of sevenless and the uptake of tracers were blocked. In addition, endosomal organization and the distribution of clathrin were greatly disrupted in Rme-8 cells, suggesting that Rme-8 participates in a clathrin-dependent process. The phenotypes of Rme-8 mutants bear a strong resemblance to those of Hsc70-4, suggesting that these two genes act in a common pathway. Indeed, biochemical and genetic data demonstrated that Rme-8 interacts specifically with Hsc70-4 via its J-domain. Thus, Rme-8 appears to function as an unexpected but critical cochaperone with Hsc70 in endocytosis. Because Hsc70 is known to act in clathrin uncoating along with auxilin, another J-protein, its interaction with Rme-8 indicates that Hsc70 can act with multiple cofactors, possibly explaining its pleiotropic effects on the endocytic pathway.

Key Words: endocytosis; clathrin; Hsc70; Rme-8; J-domain

The online version of this article includes supplemental material.

Abbreviations used in this paper: Boss, Bride of sevenless; CCV, clathrin-coated vesicle; Clc, clathrin light chain; Hk, Hook; Rme, receptor-mediated endocytosis; shi, shibire; TR-avidin, Texas red–conjugated avidin.


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