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Oliver J. Gruss¹ and Isabelle Vernos²

¹ Zentrum für Molekulare Biologie der Universität Heidelberg, Heidelberg 69120, Germany
² Cell Biology and Biophysics Program, European Molecular Biology Laboratory, Heidelberg 69117, Germany

Address correspondence to Isabelle Vernos, Cell Biology and Biophysics Program, European Molecular Biology Laboratory, Meyerhofstrasse 1, Heidelberg 69117, Germany. Tel.: 49-6221-387-285. Fax: 49-6221-387-512. email: vernos{at}EMBL.de; or o.gruss{at}zmbh.uni-heidelberg.de

Abstract
Recent work has provided new insights into the mechanism of spindle assembly. Growing evidence supports a model in which the small GTPase Ran plays a central role in this process. Here, we examine the evidence for the existence of a RanGTP gradient around mitotic chromosomes and some controversial data on the role that chromosomes play in spindle assembly. We review the current knowledge on the Ran downstream targets for spindle assembly and we focus on the multiple roles of TPX2, one of the targets of RanGTP during cell division.

Abbreviations used in this paper: KLP, kinesin-like protein; MAP, microtubule associated protein; NuMA, nuclear protein of the mitotic apparatus; RanGTP, GTPase Ran in its GTP-bound form; TPX2, targeting protein for Xklp2.

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