JCB logo
CrossRef
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published 27 September 2004. doi:10.1083/jcb.200403110
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 166, Number 7, 975-981
This Article
Right arrow Full Text
Right arrow PDF (Full Text)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Miyagi, C.
Right arrow Articles by Hirano, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miyagi, C.
Right arrow Articles by Hirano, T.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Report

 STAT3 noncell-autonomously controls planar cell polarity during zebrafish convergence and extension

Chiemi Miyagi1, Susumu Yamashita1,2, Yusuke Ohba3,4, Hisayoshi Yoshizaki3, Michiyuki Matsuda3, and Toshio Hirano1,2,5

1 Department of Molecular Oncology, Graduate School of Medicine
2 Laboratory of Developmental Immunology, Graduate School of Frontier Biosciences
3 Department of Tumor Virology, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan
4 Information and Cell Function, PRESTO, JST, Saitama 332-0012, Japan
5 Laboratory for Cytokine Signaling, RIKEN Research Center for Allergy and Immunology, Kanagawa 230-0045, Japan

Address correspondence to Toshio Hirano, Dept. of Molecular Oncology (C7), Graduate School of Medicine, Osaka University, 2-2, Yamada-oka Suita, Osaka 565-0871, Japan. Tel.: 81-6-6879-3880. Fax: 81-6-6879-3889. email: hirano{at}molonc.med.osaka-u.ac.jp


Abstract
Zebrafish signal transducer and activator of transcription 3 (STAT3) controls the cell movements during gastrulation. Here, we show that noncell-autonomous activity of STAT3 signaling in gastrula organizer cells controls the polarity of neighboring cells through Dishevelled-RhoA signaling in the Wnt-planar cell polarity (Wnt-PCP) pathway. In STAT3-depleted embryos, although all the known molecules in the Wnt-PCP pathway were expressed normally, the RhoA activity in lateral mesendodermal cells was down-regulated, resulting in severe cell polarization defects in convergence and extension movements identical to Strabismus-depleted embryos. Cell-autonomous activation of Wnt-PCP signaling by {Delta}N-dishevelled rescued the defect in cell elongation, but not the orientation of lateral mesendodermal cells in STAT3-depleted embryos. The defect in the orientation could be rescued by transplantation of shield cells having noncell-autonomous activity of STAT3 signaling. These results suggest that the cells undergoing convergence and extension movement may sense the gradient of signaling molecules, which are expressed in gastrula organizer by STAT3 and noncell-autonomously activate PCP signaling in neighboring cells during zebrafish gastrulation.

Key Words: STAT3; Dishevelled; Rho; planar cell polarity; zebrafish

Abbreviations used in this paper: FRET, fluorescence resonance energy transfer; MO, morpholino oligonucleotide; PCP, planar cell polarity; STAT, signal transducer and activator of transcription.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents