Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article Full Text PDF (Full Text) Supplemental Material Index Alert me when this article is cited Citation Map Services Email this article Similar articles in this journal Similar articles in PubMed Alert me to new content in the JCB Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Sriburi, R. Articles by Brewer, J. W. Search for Related Content PubMed PubMed Citation Articles by Sriburi, R. Articles by Brewer, J. W. Social Bookmarking                      What's this?

Rungtawan Sriburi¹, Suzanne Jackowski², Kazutoshi Mori³, and Joseph W. Brewer¹

¹ Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, Maywood, IL 60153
² Protein Science Division, Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, TN 38105
³ Department of Biophysics, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan

Correspondence to Joseph W. Brewer: jbrewer{at}lumc.edu

Abstract
When the protein folding capacity of the endoplasmic reticulum (ER) is challenged, the unfolded protein response (UPR) maintains ER homeostasis by regulating protein synthesis and enhancing expression of resident ER proteins that facilitate protein maturation and degradation. Here, we report that enforced expression of XBP1(S), the active form of the XBP1 transcription factor generated by UPR-mediated splicing of XBP1 mRNA, is sufficient to induce synthesis of phosphatidylcholine, the primary phospholipid of the ER membrane. Cells overexpressing XBP1(S) exhibit elevated levels of membrane phospholipids, increased surface area and volume of rough ER, and enhanced activity of the cytidine diphosphocholine pathway of phosphatidylcholine biosynthesis. These data suggest that XBP1(S) links the mammalian UPR to phospholipid biosynthesis and ER biogenesis.

Abbreviations used in this paper: ATF, activating transcription factor; BiP, binding protein; CCT, choline cytidylyltransferase; CDP-choline, cytidine diphosphocholine; CEPT, choline/ethanolaminephosphotransferase; CK, choline kinase; CPT, cholinephosphotransferase; PtdCho, phosphatidylcholine; PtdEtn, phosphatidylethanolamine; UPR, unfolded protein response; XBP1, X-box binding protein 1; XBP1(S), XBP1 (spliced); XBP1(U), XBP1(unspliced).

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Tian, Y., Pate, C., Andreolotti, A., Wang, L., Tuomanen, E., Boyd, K., Claro, E., Jackowski, S. (2008). Cytokine secretion requires phosphatidylcholine synthesis. J. Cell Biol. 181: 945-957 [Abstract] [Full Text]  
• Lee, A.-H., Scapa, E. F., Cohen, D. E., Glimcher, L. H. (2008). Regulation of Hepatic Lipogenesis by the Transcription Factor XBP1. Science 320: 1492-1496 [Abstract] [Full Text]  
• Bechill, J., Chen, Z., Brewer, J. W., Baker, S. C. (2008). Coronavirus Infection Modulates the Unfolded Protein Response and Mediates Sustained Translational Repression. J. Virol. 82: 4492-4501 [Abstract] [Full Text]  
• Lavery, G. G., Walker, E. A., Turan, N., Rogoff, D., Ryder, J. W., Shelton, J. M., Richardson, J. A., Falciani, F., White, P. C., Stewart, P. M., Parker, K. L., McMillan, D. R. (2008). Deletion of Hexose-6-phosphate Dehydrogenase Activates the Unfolded Protein Response Pathway and Induces Skeletal Myopathy. J. Biol. Chem. 283: 8453-8461 [Abstract] [Full Text]  
• Gomez, B. P., Riggins, R. B., Shajahan, A. N., Klimach, U., Wang, A., Crawford, A. C., Zhu, Y., Zwart, A., Wang, M., Clarke, R. (2007). Human X-Box binding protein-1 confers both estrogen independence and antiestrogen resistance in breast cancer cell lines. FASEB J. 21: 4013-4027 [Abstract] [Full Text]  
• Hayashi, A., Kasahara, T., Iwamoto, K., Ishiwata, M., Kametani, M., Kakiuchi, C., Furuichi, T., Kato, T. (2007). The Role of Brain-derived Neurotrophic Factor (BDNF)-induced XBP1 Splicing during Brain Development. J. Biol. Chem. 282: 34525-34534 [Abstract] [Full Text]  
• Okunade, G. W., Miller, M. L., Azhar, M., Andringa, A., Sanford, L. P., Doetschman, T., Prasad, V., Shull, G. E. (2007). Loss of the Atp2c1 Secretory Pathway Ca2+-ATPase (SPCA1) in Mice Causes Golgi Stress, Apoptosis, and Midgestational Death in Homozygous Embryos and Squamous Cell Tumors in Adult Heterozygotes. J. Biol. Chem. 282: 26517-26527 [Abstract] [Full Text]  
• Shen, Y., Hendershot, L. M. (2007). Identification of ERdj3 and OBF-1/BOB-1/OCA-B as Direct Targets of XBP-1 during Plasma Cell Differentiation. J. Immunol. 179: 2969-2978 [Abstract] [Full Text]  
• Kubisch, C. H., Logsdon, C. D. (2007). Secretagogues differentially activate endoplasmic reticulum stress responses in pancreatic acinar cells. Am. J. Physiol. Gastrointest. Liver Physiol. 292: G1804-G1812 [Abstract] [Full Text]  
• Hewett, J. W., Tannous, B., Niland, B. P., Nery, F. C., Zeng, J., Li, Y., Breakefield, X. O. (2007). Mutant torsinA interferes with protein processing through the secretory pathway in DYT1 dystonia cells. Proc. Natl. Acad. Sci. USA 104: 7271-7276 [Abstract] [Full Text]  
• Sriburi, R., Bommiasamy, H., Buldak, G. L., Robbins, G. R., Frank, M., Jackowski, S., Brewer, J. W. (2007). Coordinate Regulation of Phospholipid Biosynthesis and Secretory Pathway Gene Expression in XBP-1(S)-induced Endoplasmic Reticulum Biogenesis. J. Biol. Chem. 282: 7024-7034 [Abstract] [Full Text]  
• Fagone, P., Sriburi, R., Ward-Chapman, C., Frank, M., Wang, J., Gunter, C., Brewer, J. W., Jackowski, S. (2007). Phospholipid Biosynthesis Program Underlying Membrane Expansion during B-lymphocyte Differentiation. J. Biol. Chem. 282: 7591-7605 [Abstract] [Full Text]  
• Little, J. L., Wheeler, F. B., Fels, D. R., Koumenis, C., Kridel, S. J. (2007). Inhibition of Fatty Acid Synthase Induces Endoplasmic Reticulum Stress in Tumor Cells. Cancer Res. 67: 1262-1269 [Abstract] [Full Text]  
• Yu, C.-Y., Hsu, Y.-W., Liao, C.-L., Lin, Y.-L. (2006). Flavivirus Infection Activates the XBP1 Pathway of the Unfolded Protein Response To Cope with Endoplasmic Reticulum Stress. J. Virol. 80: 11868-11880 [Abstract] [Full Text]  
• O'Hara, L., Han, G.-S., Peak-Chew, S., Grimsey, N., Carman, G. M., Siniossoglou, S. (2006). Control of Phospholipid Synthesis by Phosphorylation of the Yeast Lipin Pah1p/Smp2p Mg2+-dependent Phosphatidate Phosphatase. J. Biol. Chem. 281: 34537-34548 [Abstract] [Full Text]  
• Wojcik, C., Rowicka, M., Kudlicki, A., Nowis, D., McConnell, E., Kujawa, M., DeMartino, G. N. (2006). Valosin-containing Protein (p97) Is a Regulator of Endoplasmic Reticulum Stress and of the Degradation of N-End Rule and Ubiquitin-Fusion Degradation Pathway Substrates in Mammalian Cells. Mol. Biol. Cell 17: 4606-4618 [Abstract] [Full Text]  
• Orsi, A., Fioriti, L., Chiesa, R., Sitia, R. (2006). Conditions of Endoplasmic Reticulum Stress Favor the Accumulation of Cytosolic Prion Protein. J. Biol. Chem. 281: 30431-30438 [Abstract] [Full Text]  
• Marciniak, S. J., Ron, D. (2006). Endoplasmic reticulum stress signaling in disease.. Physiol. Rev. 86: 1133-1149 [Abstract] [Full Text]  
• Gunn, K. E., Brewer, J. W. (2006). Evidence That Marginal Zone B Cells Possess an Enhanced Secretory Apparatus and Exhibit Superior Secretory Activity. J. Immunol. 177: 3791-3798 [Abstract] [Full Text]  
• Jesch, S. A., Liu, P., Zhao, X., Wells, M. T., Henry, S. A. (2006). Multiple Endoplasmic Reticulum-to-Nucleus Signaling Pathways Coordinate Phospholipid Metabolism with Gene Expression by Distinct Mechanisms. J. Biol. Chem. 281: 24070-24083 [Abstract] [Full Text]  
• Hetz, C., Bernasconi, P., Fisher, J., Lee, A.-H., Bassik, M. C., Antonsson, B., Brandt, G. S., Iwakoshi, N. N., Schinzel, A., Glimcher, L. H., Korsmeyer, S. J. (2006). Proapoptotic BAX and BAK modulate the unfolded protein response by a direct interaction with IRE1alpha.. Science 312: 572-576 [Abstract] [Full Text]  
• Wrabetz, L., D'Antonio, M., Pennuto, M., Dati, G., Tinelli, E., Fratta, P., Previtali, S., Imperiale, D., Zielasek, J., Toyka, K., Avila, R. L., Kirschner, D. A., Messing, A., Feltri, M. L., Quattrini, A. (2006). Different intracellular pathomechanisms produce diverse Myelin Protein Zero neuropathies in transgenic mice.. J. Neurosci. 26: 2358-2368 [Abstract] [Full Text]  
• Zhang, K., Kaufman, R. J. (2006). The unfolded protein response: A stress signaling pathway critical for health and disease. Neurology 66: S102-S109 [Abstract] [Full Text]  
• Caron, E., Charbonneau, R., Huppe, G., Brochu, S., Perreault, C. (2005). The structure and location of SIMP/STT3B account for its prominent imprint on the MHC I immunopeptidome. Int Immunol 17: 1583-1596 [Abstract] [Full Text]  
• Tirosh, B., Iwakoshi, N. N., Glimcher, L. H., Ploegh, H. L. (2005). XBP-1 specifically promotes IgM synthesis and secretion, but is dispensable for degradation of glycoproteins in primary B cells. J. Exp. Med. 202: 505-516 [Abstract] [Full Text]  
• Ron, D., Hampton, R. Y. (2004). Membrane biogenesis and the unfolded protein response. J. Cell Biol. 167: 23-25 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents