JCB logo
BD Biosciences
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online 13 December 2004. doi:10.1083/jcb.200409026
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 167, Number 6, 1025-1035
This Article
Right arrow Full Text
Right arrow PDF (Full Text)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Plath, K.
Right arrow Articles by Panning, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Plath, K.
Right arrow Articles by Panning, B.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Article

 Developmentally regulated alterations in Polycomb repressive complex 1 proteins on the inactive X chromosome

Kathrin Plath1, Dale Talbot2, Karien M. Hamer3, Arie P. Otte3, Thomas P. Yang4, Rudolf Jaenisch1, and Barbara Panning2

1 Whitehead Institute for Biomedical Research, Cambridge, MA 02142
2 Department of Biochemistry and Biophysics, University of California, San Francisco (UCSF), San Francisco, CA 94143
3 Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, Netherlands
4 Department of Biochemistry and Molecular Biology, University of Florida College of Medicine, Gainesville, FL 32610

Correspondence to Barbara Panning: bpanning{at}biochem.ucsf.edu; or Kathrin Plath: plath{at}wi.mit.edu

Polycomb group (PcG) proteins belonging to the polycomb (Pc) repressive complexes 1 and 2 (PRC1 and PRC2) maintain homeotic gene silencing. In Drosophila, PRC2 methylates histone H3 on lysine 27, and this epigenetic mark facilitates recruitment of PRC1. Mouse PRC2 (mPRC2) has been implicated in X inactivation, as mPRC2 proteins transiently accumulate on the inactive X chromosome (Xi) at the onset of X inactivation to methylate histone H3 lysine 27 (H3-K27). In this study, we demonstrate that mPRC1 proteins localize to the Xi, and that different mPRC1 proteins accumulate on the Xi during initiation and maintenance of X inactivation in embryonic cells. The Xi accumulation of mPRC1 proteins requires Xist RNA and is not solely regulated by the presence of H3-K27 methylation, as not all cells that exhibit this epigenetic mark on the Xi show Xi enrichment of mPRC1 proteins. Our results implicate mPRC1 in X inactivation and suggest that the regulated assembly of PcG protein complexes on the Xi contributes to this multistep process.

Abbreviations used in this paper: 293, transformed human embryonic kidney 293; Bmi-1, B lymphoma Mo-MLV insertion region 1; Cbx, chromobox homologue; Eed, embryonic ectoderm development; ES, embryonic stem; E(z), enhancer of zeste; Ezh2, enhancer of zeste 2; H3-K27, histone H3 lysine 27; H3-3mK27, histone H3 tri-methyl lysine 27; MEF, transformed mouse embryonic fibroblast; mPRC1, mouse PRC1; Pc, Polycomb; PcG, Pc group; Ph, polyhomeotic; Phc, Ph-like; PRC1, Pc repressive complex 1; PRC2, Pc repressive complex 2; Psc, posterior sex combs; Ring1, really interesting gene 1; Rnf, ring finger protein; Scm, sex combs on midleg; Scmh, sex combs on midleg homologue; TS, trophoblast stem; Xi, inactive X chromosome.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents