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Published 20 December 2004. doi:10.1083/jcb.200407158
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 167, Number 6, 1137-1146
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Article

 Domains controlling cell polarity and proliferation in the Drosophila tumor suppressor Scribble

Jennifer Zeitler, Cynthia P. Hsu, Heather Dionne, and David Bilder

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720

Correspondence to David Bilder: bilder{at}socrates.berkeley.edu

Cell polarity and cell proliferation can be coupled in animal tissues, but how they are coupled is not understood. In Drosophila imaginal discs, loss of the neoplastic tumor suppressor gene scribble (scrib), which encodes a multidomain scaffolding protein, disrupts epithelial organization and also causes unchecked proliferation. Using an allelic series of mutations along with rescuing transgenes, we have identified domain requirements for polarity, proliferation control, and other Scrib functions. The leucine-rich repeats (LRR) tether Scrib to the plasma membrane, are both necessary and sufficient to organize a polarized epithelial monolayer, and are required for all proliferation control. The PDZ domains, which recruit the LRR to the junctional complex, are dispensable for overall epithelial organization. PDZ domain absence leads to mild polarity defects accompanied by moderate overproliferation, but the PDZ domains alone are insufficient to provide any Scrib function in mutant discs. We suggest a model in which Scrib, via the activity of the LRR, governs proliferation primarily by regulating apicobasal polarity.

Abbreviations used in this paper: AJ, adherens junction; Cor, Coracle; Dlg, discs-large; GLC, germ line clone; LAP, LRR and PDZ domain-containing; Lgl, lethal giant larvae; LRR, leucine-rich repeats; Nrx, Neurexin IV; nTSG, neoplastic tumor suppressor gene; PDZ, PSD-95, Dlg, ZO-1 homology; Scrib, scribble; SJ, septate junction; WT, wild type.


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