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Published 28 February 2005. doi:10.1083/jcb.200410144
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 168, Number 5, 683-689
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 Crossing over is coupled to late meiotic prophase bivalent differentiation through asymmetric disassembly of the SC

Kentaro Nabeshima1, Anne M. Villeneuve1, and Monica P. Colaiácovo1,2

1 Department of Developmental Biology and Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305
2 Department of Genetics, Harvard Medical School, Boston, MA 02115

Correspondence to Anne M. Villeneuve: villen{at}cmgm.stanford.edu


Abstract
Homologous chromosome pairs (bivalents) undergo restructuring during meiotic prophase to convert a configuration that promotes crossover recombination into one that promotes bipolar spindle attachment and localized cohesion loss. We have imaged remodeling of meiotic chromosome structures after pachytene exit in Caenorhabditis elegans. Chromosome shortening during diplonema is accompanied by coiling of chromosome axes and highly asymmetric departure of synaptonemal complex (SC) central region proteins SYP-1 and SYP-2, which diminish over most of the length of each desynapsing bivalent while becoming concentrated on axis segments distal to the single emerging chiasma. This and other manifestations of asymmetry along chromosomes are lost in synapsis-proficient crossover-defective mutants, which often retain SYP-1,2 along the full lengths of coiled diplotene axes. Moreover, a {gamma}-irradiation treatment that restores crossovers in the spo-11 mutant also restores asymmetry of SYP-1 localization. We propose that crossovers or crossover precursors serve as symmetry-breaking events that promote differentiation of subregions of the bivalent by triggering asymmetric disassembly of the SC.

Abbreviations used in this paper: DSB, double-strand break; IF, immunofluorescence; SC, synaptonemal complex.


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