JCB logo
BD Biosciences
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online 7 March 2005. doi:10.1083/jcb.200409070
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 168, Number 6, 869-874
This Article
Right arrow Full Text
Right arrow PDF (Full Text)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Andrade, Y. N.
Right arrow Articles by Valverde, M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Andrade, Y. N.
Right arrow Articles by Valverde, M. A.
Right arrowPubmed/NCBI databases
*Gene*GEO Profiles
*HomoloGene*UniGene
*Compound via MeSH
*Substance via MeSH
Hazardous Substances DB
*CALCIUM COMPOUNDS
*CALCIUM, ELEMENTAL
Related Collections
Right arrowRelated Article
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Report

 TRPV4 channel is involved in the coupling of fluid viscosity changes to epithelial ciliary activity

Yaniré N. Andrade1,2, Jacqueline Fernandes1, Esther Vázquez1, José M. Fernández-Fernández1, Maite Arniges1, Trinidad M. Sánchez2, Manuel Villalón2, and Miguel A. Valverde1

1 Grup de Fisiologia Cel·lular i Molecular, Unitat de Senyalització Cel·lular, Universitat Pompeu Fabra, Barcelona 08003, Spain
2 Departamento de Ciencias Fisiológicas, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile

Correspondence to M.A. Valverde: miguel.valverde{at}upf.edu


Abstract
Autoregulation of the ciliary beat frequency (CBF) has been proposed as the mechanism used by epithelial ciliated cells to maintain the CBF and prevent the collapse of mucociliary transport under conditions of varying mucus viscosity. Despite the relevance of this regulatory response to the pathophysiology of airways and reproductive tract, the underlying cellular and molecular aspects remain unknown. Hamster oviductal ciliated cells express the transient receptor potential vanilloid 4 (TRPV4) channel, which is activated by increased viscous load involving a phospholipase A2–dependent pathway. TRPV4-transfected HeLa cells also increased their cationic currents in response to high viscous load. This mechanical activation is prevented in native ciliated cells loaded with a TRPV4 antibody. Application of the TRPV4 synthetic ligand 4{alpha}-phorbol 12,13-didecanoate increased cationic currents, intracellular Ca2+, and the CBF in the absence of a viscous load. Therefore, TRPV4 emerges as a candidate to participate in the coupling of fluid viscosity changes to the generation of the Ca2+ signal required for the autoregulation of CBF.

Abbreviations used in this paper: 4{alpha}PDD, 4{alpha}-phorbol 12,13-didecanoate; AACOCF3, arachidonyl trifluoromethyl ketone; CBF, ciliary beat frequency; NMDG, N-methyl-D-glucamine; pBPB, 4-bromophenacyl bromide; PLA2, phospholipase A2; TRPV4, transient receptor potential vanilloid 4.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?

Related Article

Beating under pressure
Rabiya S. Tuma
J. Cell Biol. 2005 168: 849. [Full Text] [PDF]



This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents