JCB logo
R&D Systems
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published 11 April 2005. doi:10.1083/jcb.200411118
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 169, Number 1, 49-60
This Article
Right arrow Full Text
Right arrow PDF (Full Text)
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kops, G. J.P.L.
Right arrow Articles by Cleveland, D. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kops, G. J.P.L.
Right arrow Articles by Cleveland, D. W.
Related Collections
Right arrowRelated Article
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Article

 ZW10 links mitotic checkpoint signaling to the structural kinetochore

Geert J.P.L. Kops1, Yumi Kim1, Beth A.A. Weaver1, Yinghui Mao1, Ian McLeod2, John R. Yates, III2, Mitsuo Tagaya3, and Don W. Cleveland1

1 Ludwig Institute for Cancer Research and Department of Cellular and Molecular Medicine, University of California at San Diego, La Jolla, CA 92093
2 Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037
3 School of Life Science, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo, Japan

Correspondence to Don W. Cleveland: dcleveland{at}ucsd.edu

The mitotic checkpoint ensures that chromosomes are divided equally between daughter cells and is a primary mechanism preventing the chromosome instability often seen in aneuploid human tumors. ZW10 and Rod play an essential role in this checkpoint. We show that in mitotic human cells ZW10 resides in a complex with Rod and Zwilch, whereas another ZW10 partner, Zwint-1, is part of a separate complex of structural kinetochore components including Mis12 and Ndc80–Hec1. Zwint-1 is critical for recruiting ZW10 to unattached kinetochores. Depletion from human cells or Xenopus egg extracts is used to demonstrate that the ZW10 complex is essential for stable binding of a Mad1–Mad2 complex to unattached kinetochores. Thus, ZW10 functions as a linker between the core structural elements of the outer kinetochore and components that catalyze generation of the mitotic checkpoint-derived "stop anaphase" inhibitor.

G.J.P.L. Kops's present address is Dept. of Experimental Oncology, UMC Utrecht, 3584 CG Utrecht, Netherlands.

Abbreviations used in this paper: ACA, anti-centromere antiserum; CENP, centromere-associated protein; CSF, cytostatic factor; EYFP, enhanced YFP; KCR, kinetochore-containing region; LAP, localization and affinity purification; siRNA, small interfering RNA; Zwint-1, ZW10-interacting protein 1.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?

Related Article

Mapping the checkpoint
Rabiya S. Tuma
J. Cell Biol. 2005 169: 14. [Full Text] [PDF]



This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents