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Published 11 April 2005. doi:10.1083/jcb.200411056
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 169, Number 1, 61-71
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Article

 Inhibition of the anaphase-promoting complex by the Xnf7 ubiquitin ligase

Jessica B. Casaletto1, Leta K. Nutt1, Qiju Wu1, Jonathan D. Moore1, Laurence D. Etkin2, Peter K. Jackson3,4, Tim Hunt5, and Sally Kornbluth1

1 Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710
2 Department of Molecular Genetics, University of Texas M.D. Anderson Cancer Center, Houston, TX 77030
3 Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305
4 Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305
5 Cancer Research UK, Clare Hall Laboratories, South Mimms, Herts EN6 3LD, England, UK

Correspondence to S. Kornbluth: kornb001{at}mc.duke.edu

Degradation of specific protein substrates by the anaphase-promoting complex/cyclosome (APC) is critical for mitotic exit. We have identified the protein Xenopus nuclear factor 7 (Xnf7) as a novel APC inhibitor able to regulate the timing of exit from mitosis. Immunodepletion of Xnf7 from Xenopus laevis egg extracts accelerated the degradation of APC substrates cyclin B1, cyclin B2, and securin upon release from cytostatic factor arrest, whereas excess Xnf7 inhibited APC activity. Interestingly, Xnf7 exhibited intrinsic ubiquitin ligase activity, and this activity was required for APC inhibition. Unlike other reported APC inhibitors, Xnf7 did not associate with Cdc20, but rather bound directly to core subunits of the APC. Furthermore, Xnf7 was required for spindle assembly checkpoint function in egg extracts. These data suggest that Xnf7 is an APC inhibitor able to link spindle status to the APC through direct association with APC core components.

Abbreviations used in this paper: APC, anaphase-promoting complex/cyclosome; CRS, cytoplasmic retention sequence; CSF, cytostatic factor; IAP, inhibitor of apoptosis; RRL, rabbit reticulocyte lysate; Xnf7, Xenopus nuclear factor 7.


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