JCB logo
Avanti Polar Lipids
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online 2 May 2005. doi:10.1083/jcb.200411013
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 169, Number 3, 405-413
This Article
Right arrow Full Text
Right arrow PDF (Full Text)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Walker, W.
Right arrow Articles by Hurlin, P. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Walker, W.
Right arrow Articles by Hurlin, P. J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Article

 Mnt–Max to Myc–Max complex switching regulates cell cycle entry

William Walker1, Zi-Qiang Zhou1,2, Sara Ota1, Anthony Wynshaw-Boris3,4, and Peter J. Hurlin1,2

1 Shriners Hospitals for Children, Oregon Health and Sciences University, Portland, OR 97201
2 Department of Cell and Developmental Biology, Oregon Health and Sciences University, Portland, OR 97201
3 Department of Pediatrics, University of California, San Diego, School of Medicine, La Jolla, San Diego, CA 92093
4 Department of Medicine, University of California, San Diego, School of Medicine, La Jolla, San Diego, CA 92093

Correspondence to Peter J. Hurlin: pjh{at}shcc.org

The c-Myc oncoprotein is strongly induced during the G0 to S-phase transition and is an important regulator of cell cycle entry. In contrast to c-Myc, the putative Myc antagonist Mnt is maintained at a constant level during cell cycle entry. Mnt and Myc require interaction with Max for specific DNA binding at E-box sites, but have opposing transcriptional activities. Here, we show that c-Myc induction during cell cycle entry leads to a transient decrease in Mnt–Max complexes and a transient switch in the ratio of Mnt–Max to c-Myc–Max on shared target genes. Mnt overexpression suppressed cell cycle entry and cell proliferation, suggesting that the ratio of Mnt–Max to c-Myc–Max is critical for cell cycle entry. Furthermore, simultaneous Cre-Lox mediated deletion of Mnt and c-Myc in mouse embryo fibroblasts rescued the cell cycle entry and proliferative block caused by c-Myc ablation alone. These results demonstrate that Mnt-Myc antagonism plays a fundamental role in regulating cell cycle entry and proliferation.

Abbreviations used in this paper: ChIP, chromatin immunoprecipitation; MEF, mouse embryo fibroblast.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents