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Published 9 May 2005. doi:10.1083/jcb.200411132
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 169, Number 3, 447-457
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Article

 Kinesin-1 mediates translocation of the meiotic spindle to the oocyte cortex through KCA-1, a novel cargo adapter

Hsin-ya Yang1, Paul E. Mains2, and Francis J. McNally1

1 Section of Molecular and Cellular Biology, University of California, Davis, Davis, CA 95616
2 Department of Biochemistry and Molecular Biology, Genes and Development Research Group, University of Calgary, Calgary, Alberta T2N 4N1 Canada

Correspondence to Francis J. McNally: fjmcnally{at}ucdavis.edu

In animals, female meiotic spindles are attached to the egg cortex in a perpendicular orientation at anaphase to allow the selective disposal of three haploid chromosome sets into polar bodies. We have identified a complex of interacting Caenorhabditis elegans proteins that are involved in the earliest step in asymmetric positioning of anastral meiotic spindles, translocation to the cortex. This complex is composed of the kinesin-1 heavy chain orthologue, UNC-116, the kinesin light chain orthologues, KLC-1 and -2, and a novel cargo adaptor, KCA-1. Depletion of any of these subunits by RNA interference resulted in meiosis I metaphase spindles that remained stationary at a position several micrometers from the cell cortex during the time when wild-type spindles translocated to the cortex. After this prolonged stationary period, unc-116(RNAi) spindles moved to the cortex through a partially redundant mechanism that is dependent on the anaphase-promoting complex. This study thus reveals two sequential mechanisms for translocating anastral spindles to the oocyte cortex.

Abbreviations used in this paper: APC, anaphase-promoting complex; RNAi, RNA interference.


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