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Published 20 June 2005. doi:10.1083/jcb.200412143
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 169, Number 6, 897-908
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Article

 Septin-dependent compartmentalization of the endoplasmic reticulum during yeast polarized growth

Cosima Luedeke1, Stéphanie Buvelot Frei1, Ivo Sbalzarini2, Heinz Schwarz3, Anne Spang4, and Yves Barral1

1 Biology Department, Institute of Biochemistry, Swiss Federal Institute of Technology (ETH), ETH-Hönggerberg, 8093 Zürich, Switzerland
2 Institute of Computational Science, ETH, ETH-Zentrum, 8092 Zürich, Switzerland
3 Max Planck Institute of Developmental Biology, D-72076 Tübingen, Germany
4 Friedrich Miescher Laboratory of the Max Planck Society, D-72076 Tübingen, Germany

Correspondence to Yves Barral: yves.barral{at}bc.biol.ethz.ch

Polarized cells frequently use diffusion barriers to separate plasma membrane domains. It is unknown whether diffusion barriers also compartmentalize intracellular organelles. We used photobleaching techniques to characterize protein diffusion in the yeast endoplasmic reticulum (ER). Although a soluble protein diffused rapidly throughout the ER lumen, diffusion of ER membrane proteins was restricted at the bud neck. Ultrastructural studies and fluorescence microscopy revealed the presence of a ring of smooth ER at the bud neck. This ER domain and the restriction of diffusion for ER membrane proteins through the bud neck depended on septin function. The membrane-associated protein Bud6 localized to the bud neck in a septin-dependent manner and was required to restrict the diffusion of ER membrane proteins. Our results indicate that Bud6 acts downstream of septins to assemble a fence in the ER membrane at the bud neck. Thus, in polarized yeast cells, diffusion barriers compartmentalize the ER and the plasma membrane along parallel lines.

C. Luedeke and S. Buvelot Frei contributed equally to this paper.

Abbreviations used in this paper: FLIP, fluorescence loss in photobleaching; SDK, septin-dependent kinase; WT, wild type.


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