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Published 24 October 2005. doi:10.1083/jcb.200506124
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 171, Number 2, 267-279
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Article

 A complex containing the Sm protein CAR-1 and the RNA helicase CGH-1 is required for embryonic cytokinesis in Caenorhabditis elegans

Anjon Audhya1, Francie Hyndman1, Ian X. McLeod2, Amy S. Maddox1, John R. Yates, III2, Arshad Desai1, and Karen Oegema1

1 Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093
2 Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037

Correspondence to Anjon Audhya: aaudhya{at}ucsd.edu; or Karen Oegema: koegema{at}ucsd.edu

Cytokinesis completes cell division and partitions the contents of one cell to the two daughter cells. Here we characterize CAR-1, a predicted RNA binding protein that is implicated in cytokinesis. CAR-1 localizes to germline-specific RNA-containing particles and copurifies with the essential RNA helicase, CGH-1, in an RNA-dependent fashion. The atypical Sm domain of CAR-1, which directly binds RNA, is dispensable for CAR-1 localization, but is critical for its function. Inhibition of CAR-1 by RNA-mediated depletion or mutation results in a specific defect in embryonic cytokinesis. This cytokinesis failure likely results from an anaphase spindle defect in which interzonal microtubule bundles that recruit Aurora B kinase and the kinesin, ZEN-4, fail to form between the separating chromosomes. Depletion of CGH-1 results in sterility, but partially depleted worms produce embryos that exhibit the CAR-1–depletion phenotype. Cumulatively, our results suggest that CAR-1 functions with CGH-1 to regulate a specific set of maternally loaded RNAs that is required for anaphase spindle structure and cytokinesis.

Abbreviations used in this paper: dsRNA, double-stranded RNA; DIC, differential interference contrast; RNAi, RNA-mediated interference.


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