JCB logo
Track the topics, authors and articles important to you
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online 12 December 2005. doi:10.1083/jcb.200508184
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 171, Number 6, 1035-1044
This Article
Right arrow Full Text
Right arrow PDF (Full Text)
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shang, Y.
Right arrow Articles by Gorovsky, M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shang, Y.
Right arrow Articles by Gorovsky, M. A.
Related Collections
Right arrowRelated Article
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Article

 Mutational analyses reveal a novel function of the nucleotide-binding domain of {gamma}-tubulin in the regulation of basal body biogenesis

Yuhua Shang, Che-Chia Tsao, and Martin A. Gorovsky

Department of Biology, University of Rochester, Rochester, NY 14627

Correspondence to Martin A. Gorovsky: goro{at}mail.rochester.edu

We have used in vitro mutagenesis and gene replacement to study the function of the nucleotide-binding domain (NBD) of {gamma}-tubulin in Tetrahymena thermophila. In this study, we show that the NBD has an essential function and that point mutations in two conserved residues lead to over-production and mislocalization of basal body (BB) assembly. These results, coupled with previous studies (Dammermann, A., T. Muller-Reichert, L. Pelletier, B. Habermann, A. Desai, and K. Oegema. 2004. Dev. Cell. 7:815–829; La Terra, S., C.N. English, P. Hergert, B.F. McEwen, G. Sluder, and A. Khodjakov. 2005. J. Cell Biol. 168:713–722), suggest that to achieve the precise temporal and spatial regulation of BB/centriole assembly, the initiation activity of {gamma}-tubulin is normally suppressed by a negative regulatory mechanism that acts through its NBD.

Abbreviations used in this paper: BB, basal body; GDP, guanosine diphosphate; KF, kinetodesmal fiber; ML, M loop; MT, microtubule; MTOC, MT-organizing center; NBD, nucleotide-binding domain; OA, oral apparatus.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?

Related Article

Regulating basal body replication
Rabiya S. Tuma
J. Cell Biol. 2005 171: 913. [Full Text] [PDF]





  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents