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Published online 3 April 2006. doi:10.1083/jcb.200512129
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 173, Number 1, 27-33
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 Mcp5, a meiotic cell cortex protein, is required for nuclear movement mediated by dynein and microtubules in fission yeast

Takamune T. Saito, Daisuke Okuzaki, and Hiroshi Nojima

Department of Molecular Genetics, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan

Correspondence to Hiroshi Nojima: snj-0212{at}biken.osaka-u.ac.jp

During meiotic prophase I of the fission yeast Schizosaccharomyces pombe, oscillatory nuclear movement occurs. This promotes homologous chromosome pairing and recombination and involves cortical dynein, which plays a pivotal role by generating a pulling force with the help of an unknown dynein anchor. We show that Mcp5, the homologue of the budding yeast dynein anchor Num1, may be this putative dynein anchor. mcp5+ is predominantly expressed during meiotic prophase, and GFP-Mcp5 localizes at the cell cortex. Moreover, the mcp5{Delta} strain lacks the oscillatory nuclear movement. Accordingly, homologous pairing and recombination rates of the mcp5{Delta} strain are significantly reduced. Furthermore, the cortical localization of dynein heavy chain 1 appears to be reduced in mcp5{Delta} cells. Finally, the full function of Mcp5 requires its coiled-coil and pleckstrin homology (PH) domains. Our results suggest that Mcp5 localizes at the cell cortex through its PH domain and functions as a dynein anchor, thereby facilitating nuclear oscillation.

Abbreviations used in this paper: Dhc, dynein heavy chain; EMM2, Edinburgh minimal medium 2; EMM2-N, EMM2-nitrogen; PH, pleckstrin homology; SPB, spindle pole body; WT, wild-type.


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