JCB logo
PeproTech: Your source for Cell Biology Research Reagents
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published 17 July 2006. doi:10.1083/jcb.200605028
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 174, Number 2, 245-253
This Article
Right arrow Full Text
Right arrow PDF (Full Text)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nagata, Y.
Right arrow Articles by Zammit, P. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nagata, Y.
Right arrow Articles by Zammit, P. S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Article

 Entry of muscle satellite cells into the cell cycle requires sphingolipid signaling

Yosuke Nagata1, Terence A. Partridge2, Ryoichi Matsuda1, and Peter S. Zammit3

1 Department of Life Sciences, The University of Tokyo, Meguro-ku, Tokyo 153-8902, Japan
2 Children's National Medical Center, Washington, DC 20010
3 Randall Division of Cell and Molecular Biophysics, King's College London, London SE1 1UL, England, UK

Correspondence to Peter S. Zammit: peter.zammit{at}kcl.ac.uk

Adult skeletal muscle is able to repeatedly regenerate because of the presence of satellite cells, a population of stem cells resident beneath the basal lamina that surrounds each myofiber. Little is known, however, of the signaling pathways involved in the activation of satellite cells from quiescence to proliferation, a crucial step in muscle regeneration. We show that sphingosine-1-phosphate induces satellite cells to enter the cell cycle. Indeed, inhibiting the sphingolipid-signaling cascade that generates sphingosine-1-phosphate significantly reduces the number of satellite cells able to proliferate in response to mitogen stimulation in vitro and perturbs muscle regeneration in vivo. In addition, metabolism of sphingomyelin located in the inner leaflet of the plasma membrane is probably the main source of sphingosine-1-phosphate used to mediate the mitogenic signal. Together, our observations show that sphingolipid signaling is involved in the induction of proliferation in an adult stem cell and a key component of muscle regeneration.

Abbreviations used in this paper: bSMase, bacterial sphingomyelinase; CFCS, charcoal-stripped FCS; DMS, N,N-dimethylsphingosine; eMyHC, embryonic myosin heavy chain; FB1, fumonisin B1; HGF, hepatocyte growth factor; N-SMase, neutral sphingomyelinase; PCNA, proliferating cell nuclear antigen; S1P, sphingosine-1-phosphate; TA, tibialis anterior.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents