JCB logo
Keystone Symposia
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published 11 September 2006. doi:10.1083/jcb.200512066
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 174, Number 6, 827-838
This Article
Right arrow Full Text
Right arrow PDF (Full Text)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lee, J.-A
Right arrow Articles by Kaang, B.-K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lee, J.-A
Right arrow Articles by Kaang, B.-K.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Article

 PKA-activated ApAF–ApC/EBP heterodimer is a key downstream effector of ApCREB and is necessary and sufficient for the consolidation of long-term facilitation

Jin-A Lee1, Sue-Hyun Lee1, Changhoon Lee1, Deok-Jin Chang1, Yong Lee1, Hyoung Kim1, Ye-Hwang Cheang1, Hyoung-Gon Ko1, Yong-Seok Lee1, Heejung Jun1, Dusan Bartsch2, Eric R. Kandel3, and Bong-Kiun Kaang1

1 Institute of Molecular Biology and Genetics, RIO, Department of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul 151-747, Korea
2 Department of Molecular Biology, Central Institute of Mental Health, J5, 68 159 Mannheim, Germany
3 Center for Neurobiology and Behavior, College of Physicians and Surgeons, Columbia University, New York, NY 10032

Correspondence to Bong-Kiun Kaang: kaang{at}snu.ac.kr

Long-term memory requires transcriptional regulation by a combination of positive and negative transcription factors. Aplysia activating factor (ApAF) is known to be a positive transcription factor that forms heterodimers with ApC/EBP and ApCREB2. How these heterodimers are regulated and how they participate in the consolidation of long-term facilitation (LTF) has not, however, been characterized. We found that the functional activation of ApAF required phosphorylation of ApAF by PKA on Ser-266. In addition, ApAF lowered the threshold of LTF by forming a heterodimer with ApCREB2. Moreover, once activated by PKA, the ApAF–ApC/EBP heterodimer transactivates enhancer response element–containing genes and can induce LTF in the absence of CRE- and CREB-mediated gene expression. Collectively, these results suggest that PKA-activated ApAF–ApC/EBP heterodimer is a core downstream effector of ApCREB in the consolidation of LTF.

J.-A. Lee and S.-H. Lee contributed equally to this paper.

Abbreviations used in this paper: ANOVA, analysis of variance; ApAF, Aplysia activating factor; CRE, cAMP response element; ds, double stranded; EPSP, excitatory postsynaptic potential; ERE, enhancer response element; HT, hydroxytryptamine; LTF, long-term facilitation; PKA, protein kinase A; STF, short-term facilitation; WT, wild-type.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents