JCB logo
BD Biosciences
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online 18 September 2006. doi:10.1083/jcb.200605004
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 174, Number 7, 997-1007
This Article
Right arrow Full Text
Right arrow PDF (Full Text)
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Czibener, C.
Right arrow Articles by Andrews, N. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Czibener, C.
Right arrow Articles by Andrews, N. W.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Article

 Ca2+ and synaptotagmin VII–dependent delivery of lysosomal membrane to nascent phagosomes

Cecilia Czibener1, Nathan M. Sherer1, Steven M. Becker1, Marc Pypaert2, Enfu Hui3,4, Edwin R. Chapman3,4, Walther Mothes1, and Norma W. Andrews1,2

1 Section of Microbial Pathogenesis and 2 Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06510
3 Howard Hughes Medical Institute and 4 Department of Physiology, University of Wisconsin, Madison, WI 53706

Correspondence to Norma W. Andrews: norma.andrews{at}yale.edu

Synaptotagmin (Syt) VII is a ubiquitously expressed member of the Syt family of Ca2+ sensors. It is present on lysosomes in several cell types, where it regulates Ca2+-dependent exocytosis. Because [Ca2+]i and exocytosis have been associated with phagocytosis, we investigated the phagocytic ability of macrophages from Syt VII–/– mice. Syt VII–/– macrophages phagocytose normally at low particle/cell ratios but show a progressive inhibition in particle uptake under high load conditions. Complementation with Syt VII rescues this phenotype, but only when functional Ca2+-binding sites are retained. Reinforcing a role for Syt VII in Ca2+-dependent phagocytosis, particle uptake in Syt VII–/– macrophages is significantly less dependent on [Ca2+]i. Syt VII is concentrated on peripheral domains of lysosomal compartments, from where it is recruited to nascent phagosomes. Syt VII recruitment is rapidly followed by the delivery of Lamp1 to phagosomes, a process that is inhibited in Syt VII–/– macrophages. Thus, Syt VII regulates the Ca2+-dependent mobilization of lysosomes as a supplemental source of membrane during phagocytosis.

Abbreviations used in this paper: BMM, bone marrow macrophage; PC, phosphatidylcholine; PS, phosphatidylserine; Syt, synaptotagmin; VAMP, vesicle-associated membrane protein.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents