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Published online 11 December 2006. doi:10.1083/jcb.200606062
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 175, Number 6, 1005-1015
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Article

 Neural crest–derived cells with stem cell features can be traced back to multiple lineages in the adult skin

Christine E. Wong1, Christian Paratore1, María T. Dours-Zimmermann2, Ariane Rochat3, Thomas Pietri4, Ueli Suter1, Dieter R. Zimmermann2, Sylvie Dufour4, Jean Paul Thiery4, Dies Meijer5, Friedrich Beermann6, Yann Barrandon3, and Lukas Sommer1

1 Department of Biology, Institute of Cell Biology, Swiss Federal Institute of Technology (ETH) Zurich, CH-8093 Zurich, Switzerland
2 Molecular Biology Laboratory, Department of Pathology, University Hospital of Zurich, CH-8091 Zurich, Switzerland
3 Laboratory of Stem Cell Dynamics, School of Life Sciences, Ecole Polytechnique Féderale de Lausanne and Lausanne University Hospital, CH-1015 Lausanne, Switzerland
4 UMR144, Centre National de la Recherche Scientifique, Institut Curie, 75248 Paris, Cedex 05, France
5 Department of Cell Biology and Genetics, Erasmus University Medical Center, 3000DR Rotterdam, Netherlands
6 Swiss Institute for Experimental Cancer Research, 1066 Epalinges, Switzerland

Correspondence to Lukas Sommer: sommer{at}cell.biol.ethz.ch

Given their accessibility, multipotent skin-derived cells might be useful for future cell replacement therapies. We describe the isolation of multipotent stem cell–like cells from the adult trunk skin of mice and humans that express the neural crest stem cell markers p75 and Sox10 and display extensive self-renewal capacity in sphere cultures. To determine the origin of these cells, we genetically mapped the fate of neural crest cells in face and trunk skin of mouse. In whisker follicles of the face, many mesenchymal structures are neural crest derived and appear to contain cells with sphere-forming potential. In the trunk skin, however, sphere-forming neural crest–derived cells are restricted to the glial and melanocyte lineages. Thus, self-renewing cells in the adult skin can be obtained from several neural crest derivatives, and these are of distinct nature in face and trunk skin. These findings are relevant for the design of therapeutic strategies because the potential of stem and progenitor cells in vivo likely depends on their nature and origin.

C.E. Wong and C. Paratore contributed equally to this paper.

T. Pietri's present address is Institute of Neuroscience, University of Oregon, Eugene, OR 97403.

Abbreviations used in this paper: BMP, bone morphogenic protein; CNS, central nervous system; FN, fibronectin; GFAP, glial fibrillary acidic protein; GM, growth medium; NCSC, neural crest stem cell; NRG, neuregulin; SKP, skin-derived precursor cell; SMA, smooth muscle actin.


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