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Published online January 16, 2007
doi:10.1083/jcb.200607073
The Journal of Cell Biology, Vol. 176, No. 2, 173-182
The Rockefeller University Press, 0021-9525 $30.00
© 2007 Uetake et al.
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Article

 Cell cycle progression and de novo centriole assembly after centrosomal removal in untransformed human cells

Yumi Uetake1, Jadranka Loncarek2, Joshua J. Nordberg1, Christopher N. English1, Sabrina La Terra2, Alexey Khodjakov2, and Greenfield Sluder1

1 Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01605
2 Wadsworth Center, New York State Department of Health, Albany, NY 12201

Correspondence to Greenfield Sluder: Greenfield.sluder{at}umassmed.edu

How centrosome removal or perturbations of centrosomal proteins leads to G1 arrest in untransformed mammalian cells has been a mystery. We use microsurgery and laser ablation to remove the centrosome from two types of normal human cells. First, we find that the cells assemble centrioles de novo after centrosome removal; thus, this phenomenon is not restricted to transformed cells. Second, normal cells can progress through G1 in its entirety without centrioles. Therefore, the centrosome is not a necessary, integral part of the mechanisms that drive the cell cycle through G1 into S phase. Third, we provide evidence that centrosome loss is, functionally, a stress that can act additively with other stresses to arrest cells in G1 in a p38-dependent fashion.

Abbreviation used in this paper: HMEC, human mammary epithelial cell.


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