JCB logo
HYBRIGENICS
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online January 16, 2007
doi:10.1083/jcb.200606116
The Journal of Cell Biology, Vol. 176, No. 2, 223-230
The Rockefeller University Press, 0021-9525 $30.00
© 2007 Cibrián-Uhalte et al.
This Article
Right arrow Full Text
Right arrow PDF (Full Text)
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cibrián-Uhalte, E.
Right arrow Articles by Abdelilah-Seyfried, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cibrián-Uhalte, E.
Right arrow Articles by Abdelilah-Seyfried, S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Article

 Involvement of zebrafish Na+,K+ ATPase in myocardial cell junction maintenance

Elena Cibrián-Uhalte1, Adam Langenbacher2, Xiaodong Shu2, Jau-Nian Chen2,3,4, and Salim Abdelilah-Seyfried1

1 Max Delbrück Center for Molecular Medicine, 13125 Berlin, Germany
2 Department of Molecular, Cell, and Developmental Biology, 3 Jonsson Comprehensive Cancer Center, and 4 Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA 90095

Correspondence to Salim Abdelilah-Seyfried: salim{at}mdc-berlin.de; or Jau-Nian Chen: chenjn{at}mcdb.ucla.edu

Na+,K+ ATPase is an essential ion pump involved in regulating ionic concentrations within epithelial cells. The zebrafish heart and mind (had) mutation, which disrupts the {alpha}1B1 subunit of Na+,K+ ATPase, causes heart tube elongation defects and other developmental abnormalities that are reminiscent of several epithelial cell polarity mutants, including nagie oko (nok). We demonstrate genetic interactions between had and nok in maintaining Zonula occludens-1 (ZO-1)–positive junction belts within myocardial cells. Functional tests and pharmacological inhibition experiments demonstrate that Na+,K+ ATPase activity is positively regulated via an N-terminal phosphorylation site that is necessary for correct heart morphogenesis to occur, and that maintenance of ZO-1 junction belts requires ion pump activity. These findings suggest that the correct ionic balance of myocardial cells is essential for the maintenance of epithelial integrity during heart morphogenesis.

Abbreviations used in this paper: cmlc2, cardiac myosin light chain 2; hpf, hours postfertilization; Had, heart and mind; Has, heart and soul; MO, morpholino antisense oligonucleotide; Nok, Nagie oko; wt, wild type; ZO-1, Zonula occludens-1.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents