JCB logo
Keystone Symposia
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online February 5, 2007
doi:10.1083/jcb.200608137
The Journal of Cell Biology, Vol. 176, No. 4, 483-495
The Rockefeller University Press, 0021-9525 $30.00
© 2007 Dubreuil et al.
This Article
Right arrow Full Text
Right arrow PDF (Full Text)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dubreuil, V.
Right arrow Articles by Wilsch-Bräuninger, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dubreuil, V.
Right arrow Articles by Wilsch-Bräuninger, M.
Related Collections
Right arrowRelated Article
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Article

 Midbody and primary cilium of neural progenitors release extracellular membrane particles enriched in the stem cell marker prominin-1

Véronique Dubreuil1, Anne-Marie Marzesco1, Denis Corbeil2, Wieland B. Huttner1, and Michaela Wilsch-Bräuninger1

1 Max Planck Institute of Molecular Cell Biology and Genetics, D-01307 Dresden, Germany
2 Tissue Engineering Laboratories, Biotec, D-01307 Dresden, Germany

Correspondence to Wieland B. Huttner: huttner{at}mpi-cbg.de

Expansion of the neocortex requires symmetric divisions of neuroepithelial cells, the primary progenitor cells of the developing mammalian central nervous system. Symmetrically dividing neuroepithelial cells are known to form a midbody at their apical (rather than lateral) surface. We show that apical midbodies of neuroepithelial cells concentrate prominin-1 (CD133), a somatic stem cell marker and defining constituent of a specific plasma membrane microdomain. Moreover, these apical midbodies are released, as a whole or in part, into the extracellular space, yielding the prominin-1–enriched membrane particles found in the neural tube fluid. The primary cilium of neuroepithelial cells also concentrates prominin-1 and appears to be a second source of the prominin-1–bearing extracellular membrane particles. Our data reveal novel origins of extracellular membrane traffic that enable neural stem and progenitor cells to avoid the asymmetric inheritance of the midbody observed for other cells and, by releasing a stem cell membrane microdomain, to potentially influence the balance of their proliferation versus differentiation.

V. Dubreuil's present address is UMR 8542, Centre National de la Recherche Scientifique, Ecole Normale Supèrieure, F-75005 Paris, France.

Abbreviations used in this paper: DIC, differential interference contrast; E, embryonic day; HH, Hamburger and Hamilton; mRFP, monomeric red fluorescent protein; NE, neuroepithelial.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?

Related Article

Pinched off membrane
Nicole LeBrasseur
J. Cell Biol. 2007 176: 372a. [Full Text] [PDF]



This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents