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Published online February 12, 2007
doi:10.1083/jcb.200604167
The Journal of Cell Biology, Vol. 176, No. 4, 521-533
The Rockefeller University Press, 0021-9525 $30.00
© 2007 Shcherbakova et al.
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Article

 Organization of ß-adrenoceptor signaling compartments by sympathetic innervation of cardiac myocytes

Olga G. Shcherbakova1, Carl M. Hurt1, Yang Xiang1, Mark L. Dell'Acqua2, Qi Zhang1, Richard W. Tsien1, and Brian K. Kobilka1

1 Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 95305
2 Department of Pharmacology, University of Colorado at Denver and Health Sciences Center, Aurora, CO 80045

Correspondence to Brian Kobilka: kobilka{at}stanford.edu

The sympathetic nervous system regulates cardiac function through the activation of adrenergic receptors (ARs). ß1 and ß2ARs are the primary sympathetic receptors in the heart and play different roles in regulating cardiac contractile function and remodeling in response to injury. In this study, we examine the targeting and trafficking of ß1 and ß2ARs at cardiac sympathetic synapses in vitro. Sympathetic neurons form functional synapses with neonatal cardiac myocytes in culture. The myocyte membrane develops into specialized zones that surround contacting axons and contain accumulations of the scaffold proteins SAP97 and AKAP79/150 but are deficient in caveolin-3. The ß1ARs are enriched within these zones, whereas ß2ARs are excluded from them after stimulation of neuronal activity. The results indicate that specialized signaling domains are organized in cardiac myocytes at sites of contact with sympathetic neurons and that these domains are likely to play a role in the subtype-specific regulation of cardiac function by ß1 and ß2ARs in vivo.

Y. Xiang's current address is Department of Molecular and Integrative Physiology, University of Illinois at Urbana-Champaign, Urbana, IL 61801.

Abbreviations used in this paper: AR, adrenergic receptor; GPCR, G protein–coupled receptor; KO, knockout; nAChR, nicotinic acetylcholine receptor; SGN, sympathetic ganglion neuron.


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