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Published online April 23, 2007
doi:10.1083/jcb.200611012
The Journal of Cell Biology, Vol. 177, No. 2, 197-204
The Rockefeller University Press, 0021-9525 $30.00
© 2007 Platta et al.
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 Ubiquitination of the peroxisomal import receptor Pex5p is required for its recycling

Harald W. Platta, Fouzi El Magraoui, Daniel Schlee, Silke Grunau, Wolfgang Girzalsky, and Ralf Erdmann

Abteilung für Systembiochemie, Medizinische Fakultät der Ruhr-Universität Bochum, D-44780 Bochum, Germany

Correspondence to Ralf Erdmann: Ralf.Erdmann{at}rub.de

Pex5p, which is the import receptor for peroxisomal matrix proteins harboring a type I signal sequence (PTS1), is mono- and polyubiquitinated in Saccharomyces cerevisiae. We identified Pex5p as a molecular target for Pex4p-dependent monoubiquitination and demonstrated that either poly- or monoubiquitination of the receptor is required for the ATP-dependent release of the protein from the peroxisomal membrane to the cytosol as part of the receptor cycle. Therefore, the energy requirement of the peroxisomal import pathway has to be extended by a second ATP-dependent step, namely receptor monoubiquitination.

Abbreviations used in this paper: NEM, N-ethylmaleimide; RADAR, receptor accumulation and degradation in absence of recycling.


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