JCB logo
CrossRef
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online May 7, 2007
doi:10.1083/jcb.200611166
The Journal of Cell Biology, Vol. 177, No. 3, 425-437
The Rockefeller University Press, 0021-9525 $30.00
© 2007 Di Fiore et al.
This Article
Right arrow Full Text
Right arrow PDF (Full Text)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Di Fiore, B.
Right arrow Articles by Pines, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Di Fiore, B.
Right arrow Articles by Pines, J.
Related Collections
Right arrowRelated Article
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Article

 Emi1 is needed to couple DNA replication with mitosis but does not regulate activation of the mitotic APC/C

Barbara Di Fiore1,2 and Jonathon Pines1,2

1 Wellcome Trust/Cancer Research UK Gurdon Institute and 2 Department of Zoology, University of Cambridge, Cambridge CB2 1QN, England, UK

Correspondence to Jonathon Pines: j.pines{at}gurdon.cam.ac.uk

Ubiquitin-mediated proteolysis is critical for the alternation between DNA replication and mitosis and for the key regulatory events in mitosis. The anaphase-promoting complex/cyclosome (APC/C) is a conserved ubiquitin ligase that has a fundamental role in regulating mitosis and the cell cycle in all eukaryotes. In vertebrate cells, early mitotic inhibitor 1 (Emi1) has been proposed as an important APC/C inhibitor whose destruction may trigger activation of the APC/C at mitosis. However, in this study, we show that the degradation of Emi1 is not required to activate the APC/C in mitosis. Instead, we uncover a key role for Emi1 in inhibiting the APC/C in interphase to stabilize the mitotic cyclins and geminin to promote mitosis and prevent rereplication. Thus, Emi1 plays a crucial role in the cell cycle to couple DNA replication with mitosis, and our results also question the current view that the APC/C has to be inactivated to allow DNA replication.

Abbreviations used in this paper: APC/C, anaphase-promoting complex/cyclosome; DIC, differential interference contrast; Emi1, early mitotic inhibitor 1; IRES, internal ribosome entry site; MCM, minichromosome maintenance; NEBD, nuclear envelope breakdown; Rca, regulator of cyclin A; RPE, retinal pigment epithelium.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?

Related Article

Emi1 ensures timely mitosis
Mitch Leslie
J. Cell Biol. 2007 177: 370. [Full Text] [PDF]



This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents