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Published online June 18, 2007
doi:10.1083/jcb.200611113
The Journal of Cell Biology, Vol. 177, No. 6, 995-1004
The Rockefeller University Press, 0021-9525 $30.00
© 2007 Cheerambathur et al.
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Article

 Quantitative analysis of an anaphase B switch: predicted role for a microtubule catastrophe gradient

Dhanya K. Cheerambathur1, Gul Civelekoglu-Scholey1,2, Ingrid Brust-Mascher1, Patrizia Sommi1, Alex Mogilner2, and Jonathan M. Scholey1

1 Department of Molecular and Cellular Biology and 2 Department of Neurobiology, Physiology, and Behavior, University of California at Davis, Davis, CA 95616

Correspondence to Jonathan M. Scholey: jmscholey{at}ucdavis.edu

Anaphase B in Drosophila embryos is initiated by the inhibition of microtubule (MT) depolymerization at spindle poles, which allows outwardly sliding interpolar (ip) MTs to drive pole–pole separation. Using fluorescence recovery after photobleaching, we observed that MTs throughout the preanaphase B spindle are very dynamic and display complete recovery of fluorescence, but during anaphase B, MTs proximal to the poles stabilize and therefore display lower recovery than those elsewhere. Fluorescence microscopy of the MT tip tracker EB1 revealed that growing MT plus ends localize throughout the preanaphase B spindle but concentrate in the overlap region of interpolar MTs (ipMTs) at anaphase B onset. None of these changes occurred in the presence of nondegradable cyclin B. Modeling suggests that they depend on the establishment of a spatial gradient of MT plus-end catastrophe frequencies, decreasing toward the equator. The resulting redistribution of ipMT plus ends to the overlap zone, together with the suppression of minus-end depolymerization at the poles, could constitute a mechanical switch that initiates spindle elongation.

Abbreviations used in this paper: ipMT, interpolar MT; kMT, kinetochore MT; MT, microtubule.


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