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Published online July 16, 2007
doi:10.1083/jcb.200705094
The Journal of Cell Biology, Vol. 178, No. 2, 323-335
The Rockefeller University Press, 0021-9525 $30.00
© 2007 Nejsum et al.
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Article

 A molecular mechanism directly linking E-cadherin adhesion to initiation of epithelial cell surface polarity

Lene N. Nejsum and W. James Nelson

Department of Biological Sciences and Department of Molecular and Cellular Physiology, The James H. Clark Center, Bio-X Program, Stanford University, Stanford, CA 94305

Correspondence to W. James Nelson: wjnelson{at}stanford.edu; or Lene N. Nejsum: nejsum{at}stanford.edu

Mechanisms involved in maintaining plasma membrane domains in fully polarized epithelial cells are known, but when and how directed protein sorting and trafficking occur to initiate cell surface polarity are not. We tested whether establishment of the basolateral membrane domain and E-cadherin–mediated epithelial cell–cell adhesion are mechanistically linked. We show that the basolateral membrane aquaporin (AQP)-3, but not the equivalent apical membrane AQP5, is delivered in post-Golgi structures directly to forming cell–cell contacts where it co-accumulates precisely with E-cadherin. Functional disruption of individual components of a putative lateral targeting patch (e.g., microtubules, the exocyst, and soluble N-ethylmaleimide–sensitive factor attachment protein receptors) did not inhibit cell–cell adhesion or colocalization of the other components with E-cadherin, but each blocked AQP3 delivery to forming cell–cell contacts. Thus, components of the lateral targeting patch localize independently of each other to cell–cell contacts but collectively function as a holocomplex to specify basolateral vesicle delivery to nascent cell–cell contacts and immediately initiate cell surface polarity.

Abbreviations used in this paper: AQP, aquaporin; FLIP, fluorescence loss in photobleaching; PAGFP, photoactivated GFP; tdRFP, tandem-dimer red fluorescent protein; TIRF, total internal reflection fluorescent; t-SNARE and v-SNARE, target- and vesicle-soluble N-ethylmaleimide–sensitive factor attachment protein receptor, respectively.


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