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Published online August 6, 2007
doi:10.1083/jcb.200610112
The Journal of Cell Biology, Vol. 178, No. 4, 583-594
The Rockefeller University Press, 0021-9525 $30.00
© 2007 Stehman et al.
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Article

 NudE and NudEL are required for mitotic progression and are involved in dynein recruitment to kinetochores

Stephanie A. Stehman, Yu Chen, Richard J. McKenney, and Richard B. Vallee

Department of Pathology and Cell Biology, Columbia University, New York, NY 10032

Correspondence to Richard B. Vallee: rv2025{at}columbia.edu

NudE and NudEL are related proteins that interact with cytoplasmic dynein and LIS1. Their functional relationship and involvement in LIS1 and dynein regulation are not completely understood. We find that NudE and NudEL each localize to mitotic kinetochores before dynein, dynactin, ZW10, and LIS1 and exhibit additional temporal and spatial differences in distribution from the motor protein. Inhibition of NudE and NudEL caused metaphase arrest with misoriented chromosomes and defective microtubule attachment. Dynein and dynactin were both displaced from kinetochores by the injection of an anti-NudE/NudEL antibody. Dynein but not dynactin interacted with NudE surprisingly through the dynein intermediate and light chains but not the motor domain. Together, these results identify a common function for NudE and NudEL in mitotic progression and identify an alternative mechanism for dynein recruitment to and regulation at kinetochores.

Abbreviation used in this paper: CENP, centrosome protein.


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