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Published online August 27, 2007
doi:10.1083/jcb.200612018
The Journal of Cell Biology, Vol. 178, No. 5, 765-771
The Rockefeller University Press, 0021-9525 $30.00
© 2007 Kamasaki et al.
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 Three-dimensional arrangement of F-actin in the contractile ring of fission yeast

Tomoko Kamasaki1,2, Masako Osumi2, and Issei Mabuchi1

1 Division of Biology, School of Arts and Sciences, University of Tokyo, Tokyo 153-8902, Japan
2 Department of Material and Biological Function Sciences, School of Science, Japan Women's University, Tokyo 112-8681, Japan

Correspondence to Issei Mabuchi: issei.mabuchi{at}gakushuin.ac.jp

The contractile ring, which is required for cytokinesis in animal and yeast cells, consists mainly of actin filaments. Here, we investigate the directionality of the filaments in fission yeast using myosin S1 decoration and electron microscopy. The contractile ring is composed of around 1,000 to 2,000 filaments each around 0.6 µm in length. During the early stages of cytokinesis, the ring consists of two semicircular populations of parallel filaments of opposite directionality. At later stages, before contraction, the ring filaments show mixed directionality. We consider that the ring is initially assembled from a single site in the division plane and that filaments subsequently rearrange before contraction initiates.

T. Kamasaki's Present address is Department of Oral Histology, School of Dentistry, Showa University, Tokyo 142-8555, Japan.


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