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Published online August 27, 2007
doi:10.1083/jcb.200702154
The Journal of Cell Biology, Vol. 178, No. 5, 813-827
The Rockefeller University Press, 0021-9525 $30.00
© 2007 Lewis et al.
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Article

 A nuclear envelope protein linking nuclear pore basket assembly, SUMO protease regulation, and mRNA surveillance

Alaron Lewis1, Rachael Felberbaum2, and Mark Hochstrasser3

Departments of 1 Cell Biology; 2 Molecular, Cell, and Developmental Biology; and 3 Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520

Correspondence to Mark Hochstrasser: mark.hochstrasser{at}yale.edu

The nuclear pore complex (NPC) is both the major conduit for nucleocytoplasmic trafficking and a platform for organizing macromolecules at the nuclear envelope. We report that yeast Esc1, a non-NPC nuclear envelope protein, is required both for proper assembly of the nuclear basket, a structure extending into the nucleus from the NPC, and for normal NPC localization of the Ulp1 SUMO protease. In esc1{Delta} cells, Ulp1 and nuclear basket components Nup60 and Mlp1 no longer distribute broadly around the nuclear periphery, but co-localize in a small number of dense-staining perinuclear foci. Loss of Esc1 (or Nup60) alters SUMO conjugate accumulation and enhances ulp1 mutant defects. Similar to previous findings with Mlp1, both Esc1 and Ulp1 help retain unspliced pre-mRNAs in the nucleus. Therefore, these proteins are essential for proper nuclear basket function, which includes mRNA surveillance and regulation of SUMO protein dynamics. The results raise the possibility that NPC-localized protein desumoylation may be a key regulatory event preventing inappropriate pre-mRNA export.

A. Lewis and R. Felberbaum contributed equally to this paper.

Alaron Lewis' present address is Department of Biochemistry, University of Washington, Seattle, WA 98195.

Abbreviations used in this paper: Mlp, myosin-like protein; NPC, nuclear pore complex; SUMO, small ubiquitin-related modifier; ts, temperature sensitive; UD, Ulp catalytic domain; WT, wild type.


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