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Published online September 10, 2007
doi:10.1083/jcb.200704021
The Journal of Cell Biology, Vol. 178, No. 6, 1065-1079
The Rockefeller University Press, 0021-9525 $30.00
© 2007 Sharma et al.
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Article

 Katanin regulates dynamics of microtubules and biogenesis of motile cilia

Neeraj Sharma1, Jessica Bryant1, Dorota Wloga1, Rachel Donaldson1, Richard C. Davis1, Maria Jerka-Dziadosz2, and Jacek Gaertig1

1 Department of Cellular Biology, University of Georgia, Athens, GA 30602
2 Department of Cell Biology, M. Nencki Institute of Experimental Biology, Polish Academy of Science, 02-093 Warsaw, Poland

Correspondence to Jacek Gaertig: jgaertig{at}cb.uga.edu

The in vivo significance of microtubule severing and the mechanisms governing its spatial regulation are not well understood. In Tetrahymena, a cell type with elaborate microtubule arrays, we engineered null mutations in subunits of the microtubule-severing complex, katanin. We show that katanin activity is essential. The net effect of katanin on the polymer mass depends on the microtubule type and location. Although katanin reduces the polymer mass and destabilizes the internal network of microtubules, its activity increases the mass of ciliary microtubules. We also show that katanin reduces the levels of several types of post-translational modifications on tubulin of internal and cortical microtubules. Furthermore, katanin deficiencies phenocopy a mutation of ß-tubulin that prevents deposition of polymodifications (glutamylation and glycylation) on microtubules. We propose that katanin preferentially severs older, post-translationally modified segments of microtubules.

R.C. Davis' present address is Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523.

Abbreviations used in this paper: LM, longitudinal microtubule bundle; PTM, post-translational modification; TEM, transmission electron microscopy.


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