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Published online October 29, 2007
doi:10.1083/jcb.200705197
The Journal of Cell Biology, Vol. 179, No. 3, 423-436
The Rockefeller University Press, 0021-9525 $30.00
© 2007 Maekawa et al.
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Article

 The yeast centrosome translates the positional information of the anaphase spindle into a cell cycle signal

Hiromi Maekawa1, Claire Priest3, Johannes Lechner2, Gislene Pereira4, and Elmar Schiebel1

1 Zentrum für Molekulare Biologie and 2 Biochemie-Zentrum, Universität Heidelberg, 69120 Heidelberg, Germany
3 The Paterson Institute for Cancer Research, Manchester M20 4BX, UK
4 German Cancer Research Centre, 69120 Heidelberg, Germany

Correspondence to Gislene Pereira: g.pereira{at}dkfz-heidelberg.de; or Elmar Schiebel: e.schiebel{at}zmbh.uni-heidelberg.de

The spindle orientation checkpoint (SPOC) of budding yeast delays mitotic exit when cytoplasmic microtubules (MTs) are defective, causing the spindle to become misaligned. Delay is achieved by maintaining the activity of the Bfa1–Bub2 guanosine triphosphatase–activating protein complex, an inhibitor of mitotic exit. In this study, we show that the spindle pole body (SPB) component Spc72, a transforming acidic coiled coil–like molecule that interacts with the {gamma}-tubulin complex, recruits Kin4 kinase to both SPBs when cytoplasmic MTs are defective. This allows Kin4 to phosphorylate the SPB-associated Bfa1, rendering it resistant to inactivation by Cdc5 polo kinase. Consistently, forced targeting of Kin4 to both SPBs delays mitotic exit even when the anaphase spindle is correctly aligned. Moreover, we present evidence that Spc72 has an additional function in SPOC regulation that is independent of the recruitment of Kin4. Thus, Spc72 provides a missing link between cytoplasmic MT function and components of the SPOC.

Abbreviations used in this paper: CBB, Coomassie brilliant blue; GAP, GTPase-activating protein; MALDI-TOF, matrix-assisted laser desorption ionization–time of flight; MBP, maltose-binding protein; MEN, mitotic exit network; MT, microtubule; SPB, spindle pole body; SPOC, spindle orientation checkpoint; TACC, transforming acidic coiled coil.


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