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Published online November 12, 2007
doi:10.1083/jcb.200705146
The Journal of Cell Biology, Vol. 179, No. 4, 635-641
The Rockefeller University Press, 0021-9525 $30.00
© 2007 Slepchenko et al.
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 Switching of membrane organelles between cytoskeletal transport systems is determined by regulation of the microtubule-based transport

Boris M. Slepchenko1,2, Irina Semenova1,2, Ilya Zaliapin3, and Vladimir Rodionov1,2

1 Center for Cell Analysis and Modeling and 2 Department of Cell Biology, University of Connecticut Health Center, Farmington, CT 06032
3 Department of Mathematics and Statistics, University of Nevada, Reno, NV 89557

Correspondence to Vladimir Rodionov: rodionov{at}nso.uchc.edu

Intracellular transport of membrane organelles occurs along microtubules (MTs) and actin filaments (AFs). Although transport along each type of the cytoskeletal tracks is well characterized, the switching between the two types of transport is poorly understood because it cannot be observed directly in living cells. To gain insight into the regulation of the switching of membrane organelles between the two major transport systems, we developed a novel approach that combines live cell imaging with computational modeling. Using this approach, we measured the parameters that determine how fast membrane organelles switch back and forth between MTs and AFs (the switching rate constants) and compared these parameters during different signaling states. We show that regulation involves a major change in a single parameter: the transferring rate from AFs onto MTs. This result suggests that MT transport is the defining factor whose regulation determines the choice of the cytoskeletal tracks during the transport of membrane organelles.

Abbreviations used in this paper: AF, actin filament; MT, microtubule.


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