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Published online December 10, 2007
doi:10.1083/jcb.200705108
The Journal of Cell Biology, Vol. 179, No. 6, 1149-1162
The Rockefeller University Press, 0021-9525 $30.00
© 2007 Oishi et al.
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Article

 RMD-1, a novel microtubule-associated protein, functions in chromosome segregation in Caenorhabditis elegans

Kumiko Oishi1, Hideyuki Okano2, and Hitoshi Sawa1,3

1 Laboratory for Cell Fate Decision, RIKEN Center for Developmental Biology, Chuo-ku, Kobe 650-0047, Japan
2 Department of Physiology, Keio University School of Medicine, Shinjuku-ku, Tokyo 160-8582, Japan
3 Department of Biology, Graduate School of Science, Kobe University, Nada-ku, Kobe 657-8501, Japan

Correspondence to Hitoshi Sawa: sawa{at}cdb.riken.jp

For proper chromosome segregation, the sister kinetochores must attach to microtubules extending from the opposite spindle poles. Any errors in microtubule attachment can induce aneuploidy. In this study, we identify a novel conserved Caenorhabditis elegans microtubule-associated protein, regulator of microtubule dynamics 1 (RMD-1), that localizes to spindle microtubules and spindle poles. Depletion of RMD-1 induces severe defects in chromosome segregation, probably through merotelic attachments between microtubules and chromosomes. Although rmd-1 embryos also have a mild defect in microtubule growth, we find that mutants of the microtubule growth regulator XMAP215/ZYG-9 show much weaker segregation defects. This suggests that the microtubule growth defect in rmd-1 embryos does not cause abnormal chromosome segregation. We also see that RMD-1 interacts with aurora B in vitro. Our results suggest that RMD-1 functions in chromosome segregation in C. elegans embryos, possibly through the aurora B–mediated pathway. Human homologues of RMD-1 could also bind microtubules, which would suggest a function for these proteins in chromosome segregation during mitosis in other organisms as well.

Abbreviations used in this paper: hRMD, human RMD; MAP, microtubule-associated protein; MCAK, mitotic centromere-associated kinesin; NEBD, nuclear envelope breakdown; Psa, phasmid socket absent; rDNA, ribosomal DNA; RMD, regulator of microtubule dynamics; SNP, single-nucleotide polymorphism.


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