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Published online December 31, 2007
doi:10.1083/jcb.200705163
The Journal of Cell Biology, Vol. 179, No. 7, 1365-1373
The Rockefeller University Press, 0021-9525 $30.00
© 2007 Blower et al.
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 Genome-wide analysis demonstrates conserved localization of messenger RNAs to mitotic microtubules

Michael D. Blower1,2,3, Elma Feric2,3, Karsten Weis1, and Rebecca Heald1

1 Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720
2 Department of Genetics, Harvard Medical School, Boston, MA 02115
3 Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114

Correspondence to Michael D. Blower: blower{at}molbio.mgh.harvard.edu

RNA localization is of critical importance in many fundamental cell biological and developmental processes by regulating the spatial control of gene expression. To investigate how spindle-localized RNAs might influence mitosis, we comprehensively surveyed all messenger RNAs (mRNAs) that bound to microtubules during metaphase in both Xenopus laevis egg extracts and mitotic human cell extracts. We identify conserved classes of mRNAs that are enriched on microtubules in both human and X. laevis. Active mitotic translation occurs on X. laevis meiotic spindles, and a subset of microtubule-bound mRNAs (MT-mRNAs) associate with polyribosomes. Although many MT-mRNAs associate with polyribosomes, we find that active translation is not required for mRNA localization to mitotic microtubules. Our results represent the first genome-wide survey of mRNAs localized to a specific cytoskeletal component and suggest that microtubule localization of specific mRNAs is likely to function in mitotic regulation and mRNA segregation during cell division.

Abbreviations used in this paper: CENP-E, centromere-associated protein E; CPE, cytoplasmic polyadenylation element; CSF, cytostatic factor; GO, gene ontology; MT-mRNA, microtubule-bound mRNA; rRNA, ribosomal RNA.


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