JCB logo
PeproTech: Your source for Cell Biology Research Reagents
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online December 31, 2007
doi:10.1083/jcb.200705160
The Journal of Cell Biology, Vol. 179, No. 7, 1375-1384
The Rockefeller University Press, 0021-9525 $30.00
© 2007 Tong et al.
This Article
Right arrow Full Text
Right arrow PDF (Full Text)
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Google Scholar
Right arrow Articles by Tong, Z.
Right arrow Articles by Bi, E.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tong, Z.
Right arrow Articles by Bi, E.
Related Collections
Right arrowRelated In this Issue article
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Report

 Adjacent positioning of cellular structures enabled by a Cdc42 GTPase-activating protein–mediated zone of inhibition

Zongtian Tong1, Xiang-Dong Gao1,2, Audrey S. Howell3, Indrani Bose3, Daniel J. Lew3, and Erfei Bi1

1 Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104
2 State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China
3 Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710

Correspondence to E. Bi: ebi{at}mail.med.upenn.edu

Cells of the budding yeast Saccharomyces cerevisiae are born carrying localized transmembrane landmark proteins that guide the subsequent establishment of a polarity axis and hence polarized growth to form a bud in the next cell cycle. In haploid cells, the relevant landmark proteins are concentrated at the site of the preceding cell division, to which they recruit Cdc24, the guanine nucleotide exchange factor for the conserved polarity regulator Cdc42. However, instead of polarizing at the division site, the new polarity axis is directed next to but not overlapping that site. Here, we show that the Cdc42 guanosine triphosphatase–activating protein (GAP) Rga1 establishes an exclusion zone at the division site that blocks subsequent polarization within that site. In the absence of localized Rga1 GAP activity, new buds do in fact form within the old division site. Thus, Cdc42 activators and GAPs establish concentric zones of action such that polarization is directed to occur adjacent to but not within the previous cell division site.

Abbreviations used in this paper: GAP, GTPase-activating protein; GEF, guanine nucleotide exchange factor; PBD, p21 binding domain; SEM, scanning EM.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?

Related In this Issue article

To bud where no bud has gone before
Nicole LeBrasseur
J. Cell Biol. 2007 179: 1326. [Full Text] [PDF]



This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents