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Published online January 28, 2008
doi:10.1083/jcb.200705069
The Journal of Cell Biology, Vol. 180, No. 2, 403-415
The Rockefeller University Press, 0021-9525 $30.00
© 2008 Yang et al.
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Article

 Dimeric heat shock protein 40 binds radial spokes for generating coupled power strokes and recovery strokes of 9 + 2 flagella

Chun Yang1, Heather A. Owen2, and Pinfen Yang1

1 Department of Biological Sciences, Marquette University, Milwaukee, WI 53233
2 Department of Biological Sciences, University of Wisconsin, Milwaukee, Milwaukee, WI 53211

Correspondence to Pinfen Yang: pinfen.yang{at}marquette.edu

T-shape radial spokes regulate flagellar beating. However, the precise function and molecular mechanism of these spokes remain unclear. Interestingly, Chlamydomonas reinhardtii flagella lacking a dimeric heat shock protein (HSP) 40 at the spokehead–spokestalk juncture appear normal in length and composition but twitch actively while cells jiggle without procession, resembling a central pair (CP) mutant. HSP40– cells begin swimming upon electroporation with recombinant HSP40. Surprisingly, the rescue doesn't require the signature DnaJ domain. Furthermore, the His-Pro-Asp tripeptide that is essential for stimulating HSP70 adenosine triphosphatase diverges in candidate orthologues, including human DnaJB13. Video microscopy reveals hesitance in bend initiation and propagation as well as irregular stalling and stroke switching despite fairly normal waveform. The in vivo evidence suggests that the evolutionarily conserved HSP40 specifically transforms multiple spoke proteins into stable conformation capable of mechanically coupling the CP with dynein motors. This enables 9 + 2 cilia and flagella to bend and switch to generate alternate power strokes and recovery strokes.

Abbreviations used in this paper: CP, central pair; HPD, His-Pro-Asp; HSP, heat shock protein; PMM, paromomycin; RS, radial spokes; RSP, RS protein.


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Spokes coordinate flagella
Nicole LeBrasseur
J. Cell Biol. 2008 180: 249. [Full Text] [PDF]



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