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Published online February 4, 2008
doi:10.1083/jcb.200711031
The Journal of Cell Biology, Vol. 180, No. 3, 459-466
The Rockefeller University Press, 0021-9525 $30.00
© 2008 Zou et al.
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 A microtubule-destabilizing kinesin motor regulates spindle length and anchoring in oocytes

Jianwei Zou, Mark A. Hallen, Christine D. Yankel, and Sharyn A. Endow

Department of Cell Biology, Duke University Medical Center, Durham, NC 27710

Correspondence to Sharyn A. Endow: endow{at}duke.edu

The kinesin-13 motor, KLP10A, destabilizes microtubules at their minus ends in mitosis and binds to polymerizing plus ends in interphase, regulating spindle and microtubule dynamics. Little is known about kinesin-13 motors in meiosis. In this study, we report that KLP10A localizes to the unusual pole bodies of anastral Drosophila melanogaster oocyte meiosis I spindles as well as spindle fibers, centromeres, and cortical microtubules. We frequently observe the pole bodies attached to cortical microtubules, indicating that KLP10A could mediate spindle anchoring to the cortex via cortical microtubules. Oocytes treated with drugs that suppress microtubule dynamics exhibit spindles that are reoriented more vertically to the cortex than untreated controls. A dominant-negative klp10A mutant shows both reoriented and shorter oocyte spindles, implying that, unexpectedly, KLP10A may stabilize rather than destabilize microtubules, regulating spindle length and positioning the oocyte spindle. By altering microtubule dynamics, KLP10A could promote spindle reorientation upon oocyte activation.

Abbreviations used in this paper: NCD, nonclaret disjunctional; NT, N terminus.


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