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Published online February 11, 2008
doi:10.1083/jcb.200707206
The Journal of Cell Biology, Vol. 180, No. 3, 607-618
The Rockefeller University Press, 0021-9525 $30.00
© 2008 Ben-Yair et al.
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Article

 Notch and bone morphogenetic protein differentially act on dermomyotome cells to generate endothelium, smooth, and striated muscle

Raz Ben-Yair and Chaya Kalcheim

Department of Anatomy and Cell Biology, Hebrew University-Hadassah Medical School, Jerusalem 91120, Israel

Correspondence to Chaya Kalcheim: kalcheim{at}nn-shum.ml.huji.ac.il

We address the mechanisms underlying generation of skeletal muscle, smooth muscle, and endothelium from epithelial progenitors in the dermomyotome. Lineage analysis shows that of all epithelial domains, the lateral region is the most prolific producer of smooth muscle and endothelium. Importantly, individual labeled lateral somitic cells give rise to only endothelial or mural cells (not both), and endothelial and mural cell differentiation is driven by distinct signaling systems. Notch activity is necessary for smooth muscle production while inhibiting striated muscle differentiation, yet it does not affect initial development of endothelial cells. On the other hand, bone morphogenetic protein signaling is required for endothelial cell differentiation and/or migration but inhibits striated muscle differentiation and fails to impact smooth muscle cell production. Hence, although different mechanisms are responsible for smooth muscle and endothelium generation, the choice to become smooth versus striated muscle depends on a single signaling system. Altogether, these findings underscore the spatial and temporal complexity of lineage diversification in an apparently homogeneous epithelium.

Abbreviations used in this paper: BMP, bone morphogenetic protein; BV, blood vessel; CV, cardinal vein; DM, dermomyotome; DML, dorsomedial lip; E, embryonic day; HES, hairy and enhancer of split; ICD, intracellular domain; SMA, smooth muscle actin; VEGFr, VEGF receptor; VLL, ventrolateral lip.


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