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Published online February 18, 2008
doi:10.1083/jcb.200710025
The Journal of Cell Biology, Vol. 180, No. 4, 713-720
The Rockefeller University Press, 0021-9525 $30.00
© 2008 Aguilera-Romero et al.
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 The yeast p24 complex is required for the formation of COPI retrograde transport vesicles from the Golgi apparatus

Auxiliadora Aguilera-Romero1, Joanna Kaminska2, Anne Spang3, Howard Riezman4, and Manuel Muñiz1

1 Department of Cell Biology. University of Seville, 41012 Seville, Spain
2 Institute of Biochemistry and Biophysics PAS, 02-106 Warsaw, Poland
3 Biozentrum, University of Basel, 4056 Basel, Switzerland
4 Department of Biochemistry, University of Geneva, Sciences II, CH-1211 Genève 4, Switzerland

Correspondence to Manuel Muñiz: mmuniz{at}us.es

The p24 family members are transmembrane proteins assembled into heteromeric complexes that continuously cycle between the ER and the Golgi apparatus. These cargo proteins were assumed to play a structural role in COPI budding because of their major presence in mammalian COPI vesicles. However, this putative function has not been proved conclusively so far. Furthermore, deletion of all eight yeast p24 family members does not produce severe transport phenotypes, suggesting that the p24 complex is not essential for COPI function. In this paper we provide direct evidence that the yeast p24 complex plays an active role in retrograde transport from Golgi to ER by facilitating the formation of COPI-coated vesicles. Therefore, our results demonstrate that p24 proteins are important for vesicle formation instead of simply being a passive traveler, supporting the model in which cargo together with a small GTPase of the ARF superfamily and coat subunits act as primer for vesicle formation.

Abbreviations used in this paper: CPY, carboxypeptidase Y; UPR, unfolded protein response.


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