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Published online February 18, 2008
doi:10.1083/jcb.200709161
The Journal of Cell Biology, Vol. 180, No. 4, 739-746
The Rockefeller University Press, 0021-9525 $30.00
© 2008 Carreno et al.
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 Moesin and its activating kinase Slik are required for cortical stability and microtubule organization in mitotic cells

Sébastien Carreno1, Ilektra Kouranti2, Edith Szafer Glusman3, Margaret T. Fuller3, Arnaud Echard2, and François Payre1

1 Centre de Biologie du Développement, Université Toulouse III/Centre National de la Recherche Scientifique, Unité Mixte de Recherche 5547, 31062 Toulouse Cedex 09, France
2 Institut Curie/Centre National de la Recherche Scientifique, Unité Mixte de Recherche 144, 75248 Paris Cedex 05, France
3 Department of Developmental Biology and Genetics, School of Medicine, Stanford University, Stanford, CA 94305

Correspondence to Sébastien Carreno: carreno{at}cict.fr

Cell division requires cell shape changes involving the localized reorganization of cortical actin, which must be tightly linked with chromosome segregation operated by the mitotic spindle. How this multistep process is coordinated remains poorly understood. In this study, we show that the actin/membrane linker moesin, the single ERM (ezrin, radixin, and moesin) protein in Drosophila melanogaster, is required to maintain cortical stability during mitosis. Mitosis onset is characterized by a burst of moesin activation mediated by a Slik kinase–dependent phosphorylation. Activated moesin homogenously localizes at the cortex in prometaphase and is progressively restricted at the equator in later stages. Lack of moesin or inhibition of its activation destabilized the cortex throughout mitosis, resulting in severe cortical deformations and abnormal distribution of actomyosin regulators. Inhibiting moesin activation also impaired microtubule organization and precluded stable positioning of the mitotic spindle. We propose that the spatiotemporal control of moesin activation at the mitotic cortex provides localized cues to coordinate cortical contractility and microtubule interactions during cell division.

A. Echard and F. Payre contributed equally to this paper.

S. Carreno's present address is Institute for Research in Immunology and Cancer, Université de Montréal, Montréal, Québec H2W 1R7, Canada.

Abbreviations used in this paper: dsRNA, double-stranded RNA; F-actin, filamentous actin; p-moesin, phosphorylated moesin.


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