JCB logo
R&D Systems
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online March 10, 2008
doi:10.1083/jcb.200706028
The Journal of Cell Biology, Vol. 180, No. 5, 897-904
The Rockefeller University Press, 0021-9525 $30.00
© 2008 Kiprilov et al.
This Article
Right arrow Full Text
Right arrow PDF (Full Text)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via CrossRef
Google Scholar
Right arrow Articles by Kiprilov, E. N.
Right arrow Articles by Hirsch, R. E.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kiprilov, E. N.
Right arrow Articles by Hirsch, R. E.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Report

 Human embryonic stem cells in culture possess primary cilia with hedgehog signaling machinery

Enko N. Kiprilov1, Aashir Awan1,4,5, Romain Desprat3, Michelle Velho2, Christian A. Clement4, Anne Grete Byskov5, Claus Y. Andersen5, Peter Satir1, Eric E. Bouhassira2,3, Søren T. Christensen4, and Rhoda Elison Hirsch1,2

1 Department of Anatomy and Structural Biology, 2 Department of Medicine, and 3 Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461
4 Institute of Molecular Biology, University of Copenhagen, DK-2100 Copenhagen OE, Denmark
5 Laboratory of Reproductive Biology, Rigshospitalet, DK-2100 Copenhagen OE, Denmark

Correspondence to Rhoda Elison Hirsch: rhirsch{at}aecom.yu.edu

Human embryonic stem cells (hESCs) are potential therapeutic tools and models of human development. With a growing interest in primary cilia in signal transduction pathways that are crucial for embryological development and tissue differentiation and interest in mechanisms regulating human hESC differentiation, demonstrating the existence of primary cilia and the localization of signaling components in undifferentiated hESCs establishes a mechanistic basis for the regulation of hESC differentiation. Using electron microscopy (EM), immunofluorescence, and confocal microscopies, we show that primary cilia are present in three undifferentiated hESC lines. EM reveals the characteristic 9 + 0 axoneme. The number and length of cilia increase after serum starvation. Important components of the hedgehog (Hh) pathway, including smoothened, patched 1 (Ptc1), and Gli1 and 2, are present in the cilia. Stimulation of the pathway results in the concerted movement of Ptc1 out of, and smoothened into, the primary cilium as well as up-regulation of GLI1 and PTC1. These findings show that hESCs contain primary cilia associated with working Hh machinery.

E.N. Kiprilov and A. Awan contributed equally to this paper.

Abbreviations used in this paper: AcTb, acetylated tubulin; hESC, human embryonic stem cells; hFF, human foreskin fibroblast; Hh, hedgehog; IF, immunofluorescence; Ptc1, patched 1; SAG, Smo agonist; SEM, scanning electron microscopy; SHh, sonic hedgehog; Smo, smoothened; TEM, transmission electron microscopy; Tra-1-85, tumor rejection antigen 1-85.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?




  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents